| Type I collagen is present in soft connective tissue and bone where it represents approximately 90% of the organic matrix. Type I collagen is produced from procollagen when two teloterminal propeptides are digested from the procursor and released into the circulation. Defects in one or both alleles of the type I collagen gene causes “brittle bone” disorder (osteogenesis imperfecta). Patients suffering from such defects have low levels of fully functional type I collagen resulting in bones that break very easily. |
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| Catalog # | C7510-15D |
| Applications | Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. |
| Recommended Dilutions | ELISA: 1:16,000 |
| Western Blot: Detects human Type I Collagen only in its native triple helix form. |
| Immunohistochemistry: Used on unfixed, frozen sections of human (1, 4) and bovine (2, 3) skin. If fixation is required, acetone or ethanol is recommended. |
| Optimal dilutions to be determined by the researcher. |
| Hybridoma | NS1/1.AG4.1 myeloma cells with spleen cells from mice |
| Culture Medium | RPMI 1640 with 10% fetal calf serum. |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG1,k |
| Clone No | 6F204 |
| Host | Mouse |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide. |
| Purity | Purified by Protein A affinity chromatography. |
| Immunogen | Pepsin soluble, salt-fractionated human placenta type 1 collagen. Epitope: Located near the C-terminal of the molecule. |
| Specificity | Recognizes native human type I collagen. Does not crossreact with type II, III, V or VI collagens in ELISA or Western Blot. There is no evidence of cross-reactivity with other connnective tissue proteins (laminin, fibronectin, elastin). Species Crossreactivity: Bovine, ovine, porcine, canine and goat type I collagens. Does not react with mouse, guinea pig, feline, rabbit, squirrel, equine, kangaroo or chicken type I collagen. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
