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You are here:Home » Antibodies » Abs to Collagen » Anti -Collagen Type I

Anti -Collagen Type I

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IH
Type I collagen is present in soft connective tissue and bone where it represents approximately 90% of the organic matrix. Type I collagen is produced from procollagen when two teloterminal propeptides are digested from the procursor and released into the circulation. Defects in one or both alleles of the type I collagen gene causes “brittle bone” disorder (osteogenesis imperfecta). Patients suffering from such defects have low levels of fully functional type I collagen resulting in bones that break very easily.
Catalog #C7510-15D
ApplicationsSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionsELISA: 1:16,000
Western Blot: Detects human Type I Collagen only in its native triple helix form.
Immunohistochemistry: Used on unfixed, frozen sections of human (1, 4) and bovine (2, 3) skin. If fixation is required, acetone or ethanol is recommended.
Optimal dilutions to be determined by the researcher.
HybridomaNS1/1.AG4.1 myeloma cells with spleen cells from mice
Culture MediumRPMI 1640 with 10% fetal calf serum.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No6F204
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 15mM sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenPepsin soluble, salt-fractionated human placenta type 1 collagen. Epitope: Located near the C-terminal of the molecule.
SpecificityRecognizes native human type I collagen. Does not crossreact with type II, III, V or VI collagens in ELISA or Western Blot. There is no evidence of cross-reactivity with other connnective tissue proteins (laminin, fibronectin, elastin). Species Crossreactivity: Bovine, ovine, porcine, canine and goat type I collagens. Does not react with mouse, guinea pig, feline, rabbit, squirrel, equine, kangaroo or chicken type I collagen.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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