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You are here:Home » Antibodies » Abs to Collagen » Anti -Collagen Type I

Anti -Collagen Type I

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Highly Purified E IH RIA
Catalog #C7510-17U
ApplicationsSuitable for use in ELISA, Immunohistochemistry and RIA. Not suitable for Western Blot. Other applications not tested.
Recommended DilutionELISA: Use as capture or as a second detecting antibody in sandwich format (Apparent binding constant is ~0.1nM).
Immunohistochemistry (acetone or ethanol fixed frozen 10um sections): 10-20ug/ml, Staining is similar to that for polyclonal antibodies to interstitial collagen I. Not suitable for paraffin-embedded sections or formaldhyde fixation.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No2Q652
HostMouse
SourceHuman
Concentration~0.1mg/ml
FormSupplied as a lyophilized powder. Reconstitute with 1ml sterile distilled water or sterile 40-50% glycerol.
PurityPurified from ascites by ammonium sulfate precipitation and chromatography on DEAE-cellulose.
ImmunogenHuman collagen type I.
SpecificityRecognizes both native and heat denatured human collagen type I. No crossreactivity with albumin fractions of human serum or albumins of other animal species. Less than 5% binding to collagens II and III in ELISA, RIA or immunoblot.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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