Suitable for use in ELISA, Immunohistochemistry and RIA. Not suitable for Western Blot. Other applications have not been tested.

ELISA: Use as capture or as a second detecting antibody in sandwich format (Apparent binding constant is ~0.1nM). Immunohistochemistry (acetone or ethanol fixed frozen 10um sections): 10-20ug/ml, Staining is similar to that for polyclonal antibodies to interstitial collagen I. Not suitable for paraffin-embedded sections or formaldhyde fixation. Optimal dilutions to be determined by the researcher.

Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Human collagen type I

Supplied as a lyophilized powder from mannitol, dextran and salts. Reconstitute with 1ml sterile ddH2O.

Purified by ammonium sulfate precipitation and chromatography on DEAE-cellulose from ascites.

Recognizes both native and heat denatured human collagen type I. No crossreactivity with albumin fractions of human serum or albumins of other animal species. Less than 5% binding to collagens II and III in ELISA, RIA or immunoblot.