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Specifications

Clone Host Grade Applications Monoclonal Mouse Highly Purified E IH RIA

Catalog #	C7510-17U
Applications	Suitable for use in ELISA, Immunohistochemistry and RIA. Not suitable for Western Blot. Other applications not tested.
Recommended Dilution	ELISA: Use as capture or as a second detecting antibody in sandwich format (Apparent binding constant is ~0.1nM).
	Immunohistochemistry (acetone or ethanol fixed frozen 10um sections): 10-20ug/ml, Staining is similar to that for polyclonal antibodies to interstitial collagen I. Not suitable for paraffin-embedded sections or formaldhyde fixation.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone Type	Monoclonal
Isotype	IgG1
Clone No	2Q652
Host	Mouse
Source	Human
Concentration	~0.1mg/ml
Form	Supplied as a lyophilized powder. Reconstitute with 1ml sterile distilled water or sterile 40-50% glycerol.
Purity	Purified from ascites by ammonium sulfate precipitation and chromatography on DEAE-cellulose.
Immunogen	Human collagen type I.
Specificity	Recognizes both native and heat denatured human collagen type I. No crossreactivity with albumin fractions of human serum or albumins of other animal species. Less than 5% binding to collagens II and III in ELISA, RIA or immunoblot.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.