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You are here:Home » Antibodies » Abs to Collagen » Anti -Collagen Type III

Anti -Collagen Type III

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IH N
Pooled antisera are passed over DEAE-cellulose to produce IgG-enriched fraction, which is further subjected to absorption with immobilized total human serum proteins in order to remove non-speci c antibodies. Next, the antisera fraction is absorbed with immobilized collagen types I, II, IV and V to remove crossreactive antibodies to antigenic determinants common for various collagen types. The af nity puri ed antibody is obtained by binding to immobilized native human collagen type III (the antigen used for immunization), followed by elution with acidic buffer, neutralization, dialysis, dispensing and lyophilization. Speci city was ascertained by competition ELISA. Complete inhibition is found if the antibody was preincubated with collagen type III. Inhibition by other types of collagens is observed only at 20-50 times higher concentration.
Catalog #C7510-41C
Reconstitute with 1.0ml distilled water and add preservative if preferred.
Applications This antibody can be used for immunohistochemistry on frozen cells and sections. Suitable for dot-blotting and ELISA on native human collagen type III. Use on paraf n sections is not tested. May be diluted at least 1:20 for immunohistochemical procedures if Peroxidase labeled secondary antibodies are applied. If a FITC-labeled secondary antibody is used the antibody can be diluted 1:10.
Clone TypePolyclonal
HostRabbit
SourceHuman
Concentration0.1mg/ml (OD280nm, E1% = 14)
FormAf nity Puri ed, Lyophilized. No preservative added.
PurityPurified by ammonium sulfate precipitation and cross-absorption on immobilized other collagen types and af nity chromatography.
ImmunogenExtensively puri ed native collagen type III extracted from human skin in dilute acidic buffer after mild pepsin digestion.
SpecificityRecognizes Collagen Type III.
Important NoteThis product is intended for research use only, not for use in human, therapeutic or diagnostic applications.


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