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Anti -Collagen IV (Collagen Alpha-1(IV) Chain, Collagen IV Alpha 1 Polypeptide, Collagen Type IV Alpha 1, COL4A1, Collagen Alpha-2(IV) Chain, Collagen IV Alpha 2 Polypeptide, Collagen Type IV Alpha 2, COL4A2, Collagen alpha-3(IV) Chain, Collagen Type IV Alpha 3
Anti -Collagen IV (Collagen Alpha-1(IV) Chain, Collagen IV Alpha 1 Polypeptide, Collagen
Type IV Alpha 1, COL4A1, Collagen Alpha-2(IV) Chain, Collagen IV Alpha 2 Polypeptide,
Collagen Type IV Alpha 2, COL4A2, Collagen alpha-3(IV) Chain, Collagen Type IV Alpha 3
Pricing
For pricing information, USA customers sign in. Outside USA? Please contact your distributor for pricing. Specifications
| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Highly Purified |
E IH Gel |
|
| Human plasma proteins do not interfere with collagen binding. Collagen is located in the extracellular matrix of connective tissues. It is part of the interacting network of proteoglycans and proteins that provides a structural framework for both soft and calcified connective tissues. Type IV collagen forms a two-dimensional reticulum and is a major component of the basal lamina. | | | Catalog # | C7510-53J | | Applications | Suitable for use in Immunohistochemistry, ELISA and Dot Blot. Other applications not tested. | | Recommended Dilutions | Immunohistochemistry (Frozen): 1:20 if peroxidase labeled secondary is used. If FITC is used, dilute 1:10. Not tested on paraffin. | | ELISA: Native human Collagen IV | | Dot Blot: Native human Collagen IV | | Optimal dilutions to be determined by the researcher. | | IHC Positive Control | Human kidney, liver, skin, heart | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with 1ml sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Human | | Concentration | ~0.2mg/ml | | Form | Supplied as a lyophilized powder. Reconstitute with 1ml sterile ddH2O. | | Purity | Purified by ammonium sulfate precipitation and DEAE chromatography. | | Immunogen | Native human Collagen IV | | Specificity | Recognizes human Collagen IV. Minimal crossreactions with other collagens were only found if 5-10 times higher concentrations were used of other collagens. Does not react with fibronectin, fibrinogen or laminin. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. | | Alternate names | Collagen Of Basement Membrane Alpha 1 Chain, Collagen Of Basement Membrane Alpha 2 Chain, RP11-90L1.2 |
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