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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E B IH

Type V collagen is a minor component of the connective tissue, although it is present in many different types of connective tissue. Patients with defects in the type V collagen (Ehlers-Danlos syndrome) have weakend connective tissue characterized by hyperstrechable joints and fragile, easily bruisable skin.
Catalog #	C7510-57H
Applications	Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution	ELISA: 1:50. Binds poorly to Collagen V when tested in ELISA with Collagen V coated directly onto the microtiter well.
	Immunohistochemistry: Has been used successfully on paraffin embedded and frozen, unfixed sections of human, bovine and canine skin, on rabbit and fetal bovine cornea and of new canine tissue associated with a biomaterial implant. If fixation of tissue is required, acetone or ethanol is recommended.
	Optimal dilutions to be determined by the researcher.
Culure Medium	RPMI 1640 with 10% fetal calf serum
Hybridoma	NS1/1.Ag4.1. Female SJL/J mice, immunized i.p. with antigen adsorbed onto Al(OH)3.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone Type	Monoclonal
Isotype	IgG2a,k
Clone No	6F206
Host	Mouse
Source	Canine
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, pH 7.2, 15mM sodium azide.
Purity	Purified by Protein A/G affinity chromatography.
Immunogen	Acid-digested pepsin soluble canine type V collagen.
Specificity	Recognizes canine Collagen Type V. Does not crossreact with type I, III or VI collagens. No evidence of crossreactivity with other connective tissue proteins (laminin, fibronectin, elastin). Species Crossreactivity: human, bovine, rabbit, sheep, porcine, kangaroo. Does not react with mouse, rat, guinea pig or chicken.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.