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You are here:Home » Antibodies » Abs to Collagen » Anti -Collagen Type VII

Anti -Collagen Type VII

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Ascites E B IH IC IF
Type VII collagen fibrils are composed of three identical alpha collagen chains. It's location is restricted to the basement zone beneath stratified squamous epithelia. It functions as an anchoring fibril between the external epithelia and the underlying stroma. Mutations in the collagen V11 gene are associated with all forms of dystrophic epidermolysis bullosa.
Catalog #C7510-66F
ApplicationsSuitable for use in Immunofluorescence, ELISA, Dot Blot, Western Blot, Immunohistochemistry, Immunocytochemistry and Immunoelectron Microscopy. Other applications not tested.
Recommended DilutionsImmunofluorescence (Indirect): 1:1000 on human or other mammalian frozen sections
Immunohistochemistry (Frozen sections): 1:2000 on human tongue section fixed in acetone. Specifically stains basement membranes.
Optimal dilutions to be determined by the researcher.
Cellular LocalizationSecreted, extracellular space, extracellular matrix, basement membrane
HybridomaFusion of mouse myeloma cells and splenocytes from BALB/c mice
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No8J1054
HostMouse
SourceHuman
ConcentrationNot Determined
FormSupplied as a liquid, 15mM sodium azide.
PurityAscites
ImmunogenInsoluble fractions prepared from neonatal human foreskin epidermal cells. Epitope: Located on collagenase digested Type VII collagen (150kD), i.e. the non-helical C-terminal region of the Type VII collagen dimer.
SpecificityRecognizes intact human tissue Type VII collagen (250kD). Pepsin digestion of terminal regions leads to complete loss of reactivity. Reactivity is conserved after treatment with collagenase. No reactivity against collagen types I, II, III, IV, V, laminin and fibronectin. Reacts predominantly with the basement membrane zone of stratified squamous epithelia and ectodermally derived glands. Immunoelectron microscopy shows antibody binding localized at the inferior border of the lamina densa. Species Crossreactivity: bovine, porcine, goat, guinea pig, monkey, marmoset and sheep.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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