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You are here:Home » Antibodies » Abs to Membrane Proteins » Anti -Collectrin (TMEM27, Transmembrane protein 27 Collectrin Precursor, NX17, UNQ679, PRO1312) (Biotin)

Anti -Collectrin (TMEM27, Transmembrane protein 27 Collectrin Precursor, NX17, UNQ679,
PRO1312) (Biotin)

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Specifications

Clone Host Grade Applications
Polyclonal Sheep Affinity Purified B
Collectrin (also Transmembrane protein 27) is a 37-43kD member of the MEM27 family of proteins. It is expressed on pancreatic beta-cells where it regulates insulin secretion, and on renal tubular epithelium where it mediates amino acid transport. Mature mouse Collectrin is a 208aa type I transmembrane glycoptotein. It contains a 127aa extracellular region (aa15-141) and a 60aa cytoplasmic domain (aa163-222). Collectrin dimerizes on the cell surface, and undergoes proteolytic cleavage to generate a 26kD soluble fragment. Over aa15-144, mouse Collectrin shares 92% and 86% aa identity with rat and human Collectrin, respectively.
Catalog #C7553
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionsWestern Blot: 0.1ug/ml.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostSheep
SourceMouse
Concentration~0.2mg/ml (after reconstitution)
FormSupplied as lyophilized powder from PBS, 7.4, 50ug BSA per 1ug antibody. Labeled with Biotin. Reconstitute with 250ul sterile PBS.
PurityPurified by Immunoaffinity chromatography
ImmunogenPurified, recombinant protein corresponding to aa15-144 of mouse Collectrin extracellular domain, expressed in NSO cells (Q9ESG4).
Species Sequence HomologyRat; 92%, human; 86%
SpecificityRecognizes the extracellular domain of mouse Collectrin. Species Crossreactivity: recombinant human Collectrin, ~40% .
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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