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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E B IH

Detection of C3 deposits in tissue, cells, microorganisms and immune complexes. It should be used with positive control anti-C3b. Nonactivated, absorbed C3 contains the C3a determinant, whereas activated C3 is negative with this clone.
Catalog #	C7850-13A
Applications	Suitable for use in Western Blot, Immunohistochemistry and ELISA. Other applications have not been tested.
Recommended Dilutions	Immunohistochemistry (Frozen): 1:10, Incubate for 1hr at RT.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone Type	Monoclonal
Isotype	IgG2b
Clone No	5F201
Host	Mouse
Source	Human
Concentration	~0.05mg/ml (after reconstitution)
Form	Supplied as a lyophilized powder. Reconstitute with 1ml sterile ddH2O.
Purity	Purified by Protein A affinity chromatography.
Immunogen	Human complement component C3.
Specificity	Recognizes a human C3a-determinant (C3 alpha-chain) which is also present on native C3. Allows discrimination of ubiquitous or nonspecifically bound native C3 from biologically active C3b fragment. Negative with C3b or C. Species Crossreactivity: guinea pig and rabbit
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.