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Anti -Complement C3, C3d (Complement C3d Fragment, Complement Component C3d, C3/C3d, Complement Component 3, Complement Factor 3, CPAMD1)
Anti -Complement C3, C3d (Complement C3d Fragment, Complement Component C3d, C3/C3d,
Complement Component 3, Complement Factor 3, CPAMD1)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Goat |
Serum |
E |
|
| C3d is a component of complement. Cell-bound C3b may undergo limited proteolysis to release the fragment C3c and retain C3d on the membrane. | | | Catalog # | C7850-14Y5 | | Applications | Suitable for use in ELISA, Immunoelectrophoresis and Single and Double Radial Immunodiffusion (Ouchterlony). Other applications not tested. | | Recommended Dilution | Immunoelectrophoresis: 120ul against 2ul human plasma or equivalent | | Double Radial Immunodiffusion: Use a rosette arrangement with 10ul in 3mm diameter center well and 2ul plasma samples (neat and serially diluted) in 2mm diameter peripheral wells. | | Single Radial Immunodiffusion: use 1% antiserum in the gel | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Goat | | Source | Human | | Concentration | Not determined | | Form | Supplied as a lyophilized powder. Preservative free. Reconstitute with 1ml sterile ddH2O. Dilute further in PBS, pH 7.2. | | Purity | Serum | | Immunogen | C3d isolated and purified from pooled normal human serum. | | Specificity | Recognizes human C3d. In immunoelectrophoresis and double radial immunodiffusion against normal human serum, a single precipitin line is obtained and giving a reaction of full identity with the purified immunogen. Also reacts with fragments bearing common determinants: C3, C3b, C3bi. Does not react with any other proteins component of human serum or plasma. In supernatants of serum containing 10% polyethyleneglycol only a reaction with C3d has been obtained. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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