| Murine complement factor C4, formerly known as Gg protein, consists of an alpha-, beta-, and gamma-chain. The classical pathway of complement and the Mannose binding lecting activation pathway converge at C4. C1s, MASP-1 and MASP-2 cleave C4 resulting in the formation of C4a and C4b. the latter can be cleaved to result in C4c and C4d in which step all functional sires are lost. The C7850-16B reacts with intact C4, C4b, C4d. C4 is an acute phase protein that is produced by hepatocytes, monocytes and intestinal epithelial cells. |
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| Catalog # | C7850-16B |
| Applications | Suitable for use in ELISA, Western Blot, Immunofluorescence, Immunoprecipitation and Immunohistochemistry. Other applications not tested. |
| Recommended Dilutions | ELISA: 1:50 |
| Flow Cytometry: 1:50 |
| Western Blot: 1:50. Use non-reduced sample |
| Immunoprecipitation: 1:50 |
| mmunohistochemistry (Frozen): 1:50. Fix sections with ice-cold acetone and block with 2% BSA, FCS and PBS. |
| Optimal dilutions to be determined by the researcher. |
| Positive Control | T-cell regions of mouse spleen |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| Clone Type | Monoclonal |
| Isotype | IgG2a |
| Clone No | 6A530 |
| Host | Rat |
| Source | Mouse |
| Concentration | ~0.1mg/ml |
| Form | Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide |
| Purity | Purified by Protein G affinity chromatography. |
| Immunogen | Thymocytes decorated with Thy-1 antibody and complement components |
| Specificity | Recognizes mouse Complement C4. Reacts with intact C4, C4b and C4d. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
