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C7850-16B Rat Anti-Complement C4

Specifications
References
Clone Type
Monoclonal
Host
Rat
Source
Mouse
Isotype
IgG2a
Clone Number
6A530 (16D2)
Grade
Affinity Purified
Applications
E FC IF IHC IP WB
Crossreactivity
Mo
Shipping Temp
Blue Ice
Storage Temp
-20°C

Murine complement factor C4, formerly known as Gg protein, consists of an alpha-, beta-, and gamma-chain. The classical pathway of complement and the Mannose binding lecting activation pathway converge at C4. C1s, MASP-1 and MASP-2 cleave C4 resulting in the formation of C4a and C4b. the latter can be cleaved to result in C4c and C4d in which step all functional sires are lost. The C7850-16B reacts with intact C4, C4b, C4d. C4 is an acute phase protein that is produced by hepatocytes, monocytes and intestinal epithelial cells.

Applications
Suitable for use in ELISA, Western Blot, Immunofluorescence, Immunoprecipitation, Flow Cytometry and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
ELISA: 1:50 Western Blot: 1:50. Use non-reduced sample Immunoprecipitation: 1:50 mmunohistochemistry (Frozen): 1:300 Fix sections with 10% buffered formalin solution for 10 minutes @ RT. Immunofluorescence (Frozen): 1:50. Fix sections with ice-cold acetone and block with 2% BSA, FCS and PBS. Optimal dilutions to be determined by the researcher.
Positive Control
T-cell regions of mouse spleen
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Thymocytes decorated with Thy-1 antibody and complement components.
Form
Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes mouse Complement C4. Reacts with intact C4, C4b and C4d.
References
1. Kremmer, E, et al., Hybridoma 9(4): 309 (1990). 2. Gadjeva, M. et al., J Immunol 169:5489 (2002) 3. Paul E. et al., Eur J Imunol 32:2672 (2002) 4. Murata K et al., Am J Transplant 7: 2605 (2007)
USBio References
No references available
Conjugates
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