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Specifications

Clone Host Grade Applications Monoclonal Rat Affinity Purified B IP IH IF

Murine complement factor C4, formerly known as Gg protein, consists of an alpha-, beta-, and gamma-chain. The classical pathway of complement and the Mannose binding lecting activation pathway converge at C4. C1s, MASP-1 and MASP-2 cleave C4 resulting in the formation of C4a and C4b. the latter can be cleaved to result in C4c and C4d in which step all functional sires are lost. The C7850-16B reacts with intact C4, C4b, C4d. C4 is an acute phase protein that is produced by hepatocytes, monocytes and intestinal epithelial cells.
Catalog #	C7850-16B
Applications	Suitable for use in Western Blot, Immunoprecipitation, Immunofluorescence and Immunohistochemistry. Other applications not tested.
Recommended Dilution	Western Blot: 1:50. Non-reducing conditions
	mmunohistochemistry (frozen): 1:50. Can be fixed in ice-cold acetone and blocked with 2% BSA, FCS and PBS.
	Optimal dilutions to be determined by the researcher.
Positive Control	T-cell regions of mouse spleen
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone Type	Monoclonal
Isotype	IgG2a
Clone No	6A530
Host	Rat
Source	Mouse
Concentration	~0.1mg/ml
Form	Supplied as a liquid in PBS, 0.02% sodium azide, 0.1% BSA.
Purity	Purified
Immunogen	Complement C4
Specificity	Recognizes mouse C4. Reacts with an epitope on C4d.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.