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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E B

Catalog #	C7850-16D
C4 is a component of the classical complement pathway and is split by activated C1s to C4a and C4b. Together C2a and C4b forms the classical C3 convertase (1). The molecular mass of C4 is 204kD and it consists of 3 subunits	a (97kD), b (75kD) and g (33kD).
Applications	Suitable for use in ELISA (2), Western Blot. Other applications not tested.
Recommended Dilution	ELISA: 1:8000 (2)
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1,k
Clone No	25.3F4
Host	Mouse
Source	Human
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, pH 7.2, 15mM sodium azide.
Purity	Purified by Protein A/G affinity chromatography from culture supernatant.
Immunogen	C4 isolated from human plasma.
Specificity	Specific for human C4b. Epitope specificity differs from that of clone 6F71 as determined by inhibition ELISA. Reacts strongly with C4. A strong reaction is seen with normal human serum when tested in sandwich ELISA in combination with a polyclonal antibody against C4. No reaction is seen with C4-depleted serum. Also reacts with C4b deposited on coated antibody molecules. In Western blotting after SDS-PAGE reacts with C4 in both reduced and non-reduced forms.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.