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You are here:Home » Antibodies » Abs to Complement » Anti -Complement C4

Anti -Complement C4

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B
Catalog #C7850-16D
C4 is a component of the classical complement pathway and is split by activated C1s to C4a and C4b. Together C2a and C4b forms the classical C3 convertase (1). The molecular mass of C4 is 204kD and it consists of 3 subunits a (97kD), b (75kD) and g (33kD).
ApplicationsSuitable for use in ELISA (2), Western Blot. Other applications not tested.
Recommended DilutionELISA: 1:8000 (2)
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No25.3F4
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 15mM sodium azide.
PurityPurified by Protein A/G affinity chromatography from culture supernatant.
ImmunogenC4 isolated from human plasma.
SpecificitySpecific for human C4b. Epitope specificity differs from that of clone 6F71 as determined by inhibition ELISA. Reacts strongly with C4. A strong reaction is seen with normal human serum when tested in sandwich ELISA in combination with a polyclonal antibody against C4. No reaction is seen with C4-depleted serum. Also reacts with C4b deposited on coated antibody molecules. In Western blotting after SDS-PAGE reacts with C4 in both reduced and non-reduced forms.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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