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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E B IH IC

C5 is involved in the activation of the lythic pathway within the complement system which is an important factor in innate immunity. The complement pathways can be divided in the activation pathways and lytic pathway. The activation pathways lead via C3 to the cleavage of the fifth complement component C5 into C5a and C5b. C5b initiates the assembly of the membrane attack complex (MAC) that mediates cytolysis.
Catalog #	C7850-30A
Applications	Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended Dilution	Immunohistochemistry (frozen): 1:10. Incubation time 1hr at RT.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone Type	Monoclonal
Isotype	IgG1
Clone No	5F208
Host	Mouse
Source	Human
Concentration	~0.05mg/ml
Form	Supplied as a lyophilized powder. Reconstitute with 1ml sterile dH2O.
Purity	Purified by Protein A affinity chromatography.
Immunogen	Activated human complement component C5.
Specificity	Recognizes human C5b-9 complexes via activated C5, especially in different forms of glomerulonephritis. Does not react with native C5 (See C7850-23). Normal fresh and activated serum subjected to SDS-PAGE Western Blot (nonreduced), visualizes reactivity with the SDS-denatured C5 (200kD) abd C5b (185kD), the latter together with a faint cleavage product (60kD) only after activation.
Species Crossreactivity	Primates, bovine, porcine and goat.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.