USBio Logo

C7850-60D Mouse Anti-Complement Factor B, Ba Fragment

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Source
Human
Swiss Prot
P00751
Isotype
IgG1
Clone Number
014III-33.2.4.3
Grade
Affinity Purified
Applications
E FC IHC WB
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C

C3b associates with complement factor B, inducing conformational change. This enables complement factor D to cleave the N-terminal of complement factor B (the Ba subunit), leaving the 63 kDa Bb subunit associated with C3b, forming C3 convertase. Subunit Ba inhibits lymphocyte proliferation. Conversely, subunit Bb is involved in the proliferation of preactivated B lymphocytes.

Applications
Suitable for use in ELISA, Flow Cytometry, Functional Assays, Immunohistochemistry and Western Blot. Other applications have not been tested.
Recommended Dilutions
Immunohistochemistry: Frozen sections Optimal dilutions to be determined by the researcher.
Immunohistochemistry Positive Control
Kidney from post streptococcal glomerulonephritis patients
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Purified human complement factor B
Form
Supplied as a liquid in borate buffered saline, 0.09% sodium azide
Purity
Purified by Protein A affinity chromatography
Specificity
Recognizes the 30kD Ba fragment of the 90kD human complement factor B. Binds to both the Ba fragment and the whole protein so is not suitable for all ELISA applications.
References
1. Xu, Y. et al. (2001) Structural biology of the alternative pathway convertase. Immunol. Rev. 180:123-135, 2. Ponnuraj, K. et al. (2004) Structural analysis of engineered Bb fragment of complement factor B: insights into the activation mechanism of the alternative pathway C3-convertase. Mol. Cell. 14:17-28.3. Zipplies, J.K. et al. (2010) Complement factor B expression profile in a spontaneous uveitis model. Immunobiology. 215 (12): 949-55. 4. Katschke, K.J. Jr. et al. (2018) Classical and alternative complement activation on photoreceptor outer segments drives monocyte-dependent retinal atrophy. Sci Rep. 8 (1): 7348. 5. Loyet, K.M. et al. (2012) Activation of the alternative complement pathway in vitreous is controlled by genetics in age-related macular degeneration. Invest Ophthalmol Vis Sci. 53 (10): 6628-37.
USBio References
No references available
Conjugates
Pricing
Order
Proceed to Checkout
Cart Summary
ProductSizeListYour PriceQtyExt Price
Subtotal:Subtotal:
Subtotal:Subtotal:
Total Coupon Savings:Total Coupon Savings:()
Your cart is currently empty.
- Coupon Code
Recently Viewed
Contact Us

Visit our technical library or contact our support staff to answer your questions.

Telephone:
1-800-520-3011

Library | Contact

Distributors

For customers outside of the United States, please use one of our many distributors.

View Distributors

Payment Methods

We accept the following payment methods as well as pay-by-invoice.

MasterCard Visa PayPal
© 2023-2024 United States Biological - All Rights Reserved