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You are here:Home » Antibodies » Abs to Complement » Anti -Complement Factor B

Anti -Complement Factor B

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B
Factor B is a component of the alternative complement pathway. The protein is a single chain polypeptide with a molecular mass of 93kD. Factor B is cleaved by factor D into the Ba component (30kD) and the Bb (63kD). Bb together with C3b forms the alternative pathway C3 convertase C3bBb. Plasma concentration is approximately 200ug/ml (1).
Catalog #C7850-63
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA: 1:800.
Western Blot: Recognizes the Ba fragment of factor
B in its non-reduced form only.
Optimal dilutions to be determined by the researcher.
Culture MediumRPMI 1640 with 2-10% fetal calf serum.
HybridomaP3X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No6F81
HostMouse
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 15mM sodium azide before the addition of glycerol to 40%.
PurityPurified by Protein A/G affinity chromatography from culture supernatant.
ImmunogenComplement factor B isolated from human plasma.
SpecificityRecognizes human Complement Factor B.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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