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Anti -Connexin 31 (CX31, Gap Junction Beta 3 Protein, CXB-3)
Anti -Connexin 31 (CX31, Gap Junction Beta 3 Protein, CXB-3)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Serum |
E B |
|
| Catalog # | C7853-05B | | Applications | Suitable for use in ELISA, Western Blot. Other applications not tested. | | Recommended Dilution | Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure using ECL. An antibody raised against a GST-fusion has been shown to recognize ~31kD band in skin (3). | | ELISA (1 | 100K using 50-100ng CX31 control peptide/well. | | Immunohistochemistry & Immunofluorescence | we recommend the use of affinity purified antibody at 2-20ug/ml in formaldehyde fixed tissue (3). | | Optimal dilutions to be determined by the researcher. | | Control Peptide | C7853-61D Connexin 31, Mouse, Control Peptide (CX31, Gap Junction Beta 3 Protein, CXB-3) | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Mouse | | Concentration | As reported | | Form | Supplied as a liquid, neat serum, 40% glycerol. | | Purity | Serum | | Immunogen | A 17 amino acid peptide sequence within the 1st cytoplasmic domain of the rat Cx31 (2) was used to generate anti-Mouse Cx31. Amino terminal Cysteine was added for conjugation to KLH (carrier protein) | | Specificity | The mouse Cx31 immunogenic peptide sequence shows 3 aa differences in rat Cx 31 (4). The Cx31 peptide sequence does not significant homology with other known Connexins. Actual crossreactivity of antibodies in various species is not established | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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