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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified E B IF

Gap junctions have important functions in the transmission of signals across synapses in the nervous system (3). Connexin29 is a gap junction protein of 258 amino acids encoded by a novel single copy mouse gene that shows 75% sequence identity to human connexin 30.2 (4). Connexin29 mRNA (4.4 kb) is highly abundant in mouse sciatic nerve and is less abundant in spinal cord and adult brain (4). Expression data suggest that the new connexin gene is active in myelin-forming glial cells (4).
Catalog #	C7853-50
Applications	Suitable for use in Immunofluorescence, ELISA and Western Blot. Other applications not tested.
Recommended Dilution	Immunofluorescence: 0.5-2ug/ml
	Western Blot: 0.5-2ug/ml
	Optimal dilutions to be determined by the researcher.
Positive Control	Mouse brain or sciatic nerve homogenates
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol 40-50%, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Mouse
Concentration	0.25mg/ml
Form	Supplied as a liquid in PBS, pH 7.4 0.01% sodium azide.
Purity	Purified by immunoaffinity chromatography.
Immunogen	Synthetic peptide derived from the C-terminal sequence of mouse Cx29.
Specificity	Recognizes mouse Cx29. Peptide inhibition studies were used to con rm the antibody’s speci city. In Western Blot, two bands are usually observed. A band at ~29 kD is the connexin29 monomer, while a second band at ~58 kD corresponds to the Cx29 dimer.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.