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You are here:Home » Antibodies » Abs to Connexin, Junction Proteins » Anti -Connexin 46 (Cx46, Gap Junction Alpha 3 Protein, CXA-3)

Anti -Connexin 46 (Cx46, Gap Junction Alpha 3 Protein, CXA-3)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IH IF
Gap junctions are composed of transmembrane channels that link the cytoplasm of neighboring cells. They differ from other membrane channels since they exist between two cells. Gap junctions are relatively non-specific. They allow passive diffusion of small molecules up to 1000D. The junctions exist in almost all vertebrate and non-vertebrates cells. It is believed that gap junctions play an important for intercellular communications and affect growth and differentiation of cells. Gap junctional channel is composed of a hemichannel (connexon) in the cell membrane of one cell joined in mirror symmetry with a connexon in the opposing cell. Each connexon is an oligomer of six protein subunits that define the axial aqueous pore. Molecular cloning studies have identified a family of at least 12 highly related Connexins that are designated according to MW, Cx26-50. Hydropathy analyses of Cx sequences predicts 4 transmembrane (TM), 2 extracellular (EC) and 3 cytoplasmic (CP) domains. The EC, TM and N-terminal CP domains are well conserved among family members. The Central and C-terminal domains are highly variable in both sequence and size. The N- and C-termini are predicted to be cytoplasmic.
Catalog #C7858-07A
ApplicationsSuitable for use in ELISA, Immunohistochemistry, Immunofluorescence and Western Blot. Other applications not tested.
Recommended DilutionsWestern Blot: 1-10ug/ml; Using ECL. The protein detected may migrate slightly faster than the 46kD.
ELISA: 1:100,000. Using 50-100ng C7858-07B Control Peptide/well.
Immunohistochemistry: 2-10ug/ml. Adherent cells can be fixed in 50% methanol-50% acetone or 1% paraformaldhyde.
Optimal dilutions to be determined by the researcher
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceRat
Concentration~1mg/ml
FormSupplied as a liquid in PBS, 0.1% BSA.
PurityPurified by affinity chromatography.
ImmunogenA synthetic peptide corresponding to 11aa within the C-terminal cytoplasmic domain of rat Cx46 conjugated to KLH. Species sequence homology: mouse, 91%; human, <50%.
SpecificityRecognizes rat Cx46. No significant homology is seen with other Connexins.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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