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You are here:Home » Cloning » Cloning-CpG DNA » CpG-DNA, Mouse, BioGenomics™

CpG-DNA, Mouse, BioGenomics™

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Specifications

Bacterial DNA is known to induce acute inflammatory responses. Bacterial DNA acts as a pathogen-associated molecular pattern by virtue of a 20-fold greater frequency of unmethylated CG dinucleotides found in microbial DNA versus vertebrate DNA. Cellular activation by deoxy-cytidylate-phosphate-deoxy- guanylate (CpG)-DNA occurs via the Toll/IL-1R signal pathway. TLR9 appears to be a major component of the CpG-DNA receptor, acting by direct binding to CpG-DNA. The synthetic oligodeoxynucleotides (ODN) contain the proper CpG-DNA motif mimicing the immunostimulatory effects of bacterial DNA. The human optimal CpG motif GTCGTT differs from the optimal mouse CpG motif GACGTT. Non CpG-DNA has been shown to compete with CpG-DNA in in vitro stimulation experiments.
Catalog #C7905-85
SourceThis mouse CpG is a 20-mer ODN has the following sequence: 5'-tccatgacgttcctgatgct-3'. Regular letters represent phosphorothiorate linkage.
ApplicationsSuitable for use for biological assays in vitro to activate murine cells. Other applications not tested.
Recommended DilutionsIn vitro Stimulation: 0.01-10uM
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PurityPurified
ConcentrationNot determined
FormSupplied as a lyophilized powder. Reconstitute with dH2O.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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