Anti -CAMP (Cathelicidin Antimicrobial Peptide, 18kD Cationic Antimicrobial Protein,
CAP-18, hCAP-18, FALL-39 Peptide Antibiotic, CAP18, FALL39, HSD26)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Sheep |
Affinity Purified |
B |
|
| CAMP (Cathelicidin AntiMicrobial Peptide also18kD cationic antimicrobial protein, CAP18, LL37, FALL39 and HSD26) is a member of the cathelicidin family of proteins. It is widely expressed, being found associated with neutrophils, bronchial epithelium, renal tubule epithelium, activated keratinocytes, gd T cells, monocytes, NK cells, colonic epithelium and the stratum basale of nonkeratinized epithelium found in the vagina and oral cavity. CAMP has marked antimicrobial activity against both Gm+ and Gm- bacteria, and acts as a chemoattractant for neutrophils, monocytes and mast cells. CAMP is synthesized as 170aa preproprecursor. It contains a 30aa signal sequence, a 103aa, 14kD prosegment (aa31-131) and a 4-5kD, 37aa (aa134-170) C-terminal mature fragment (LL37) or 39aa (aa132-170) C-terminal mature fragment (FALL39). In neutrophils, the 18-19kD proprecursor is stored in granules, where, upon activation, it is enzymatically cleaved and released. While both the prosegment and C-terminal fragments possess antimicrobial activity, the prosegment also shows protease activity, while the C-terminal fragment also shows chemotactic activity. The prosegment may form homodimers, while the C-terminal fragment (LL37) is reported to form homotetramers. Over aa141-170, human CAMP shares only 50% and 57% aa sequence identity with mouse and rat CAMP, respectively. | | | Catalog # | C7906-74C | | Applications | Suitable for use in Western Blot. Other applications not tested. | | Recommended Dilution | Western Blot: 1ug/ml | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Sheep | | Source | Human | | Concentration | ~0.1mg/ml after reconstitution | | Form | Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.2mg/ml. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | Recombinant corresponding to aa134-170 from human CAMP expressed in E.coli. | | Specificity | Recognizes human CAMP. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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