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C7948-15H 11-CTP (Biotin) (g-[N-(Biotin-6-amino-hexanoyl)]-5-propargylamino-cytidine-5’-triphosphate, Triethylammonium Salt) CAS:

Specifications
References
Grade
Highly Purified
Molecular Formula
C28H44N7O17P3S
Molecular Weight
875.67
EU Commodity Code
38220090
Shipping Temp
Blue Ice
Storage Temp
-20°C

Biotin-labeled Nucleotides are used for non-fluorescent labeling of nucleic acids in purification and detection applications.

11-CTP (Biotin) (Biotin-11-cytidine-5’-triphosphate) can replace CTP for in vitro transcription reaction catalyzed by T3, T7 or SP6 RNA polymerases. The biotin-labeled RNA transcripts produced by these reactions are suitable for a wide range of applications such as nucleic acid hybridization, sequencing, and genome analysis. The transcription reaction produces multiple RNA copies of the DNA template(s) during a short incubation period. RNA probes offer higher target specificity and greater sensitivity than the corresponding DNA-DNA hybrids.
Applications
Suitable for use in Microarray based hybridization detection and Ribonuclease protection assay Other applications have not been tested.
Spectroscopic Properties
λmax 294 nm; ε 9300
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity
~95%
Concentration
~5mM
Form
Supplied as a liquid in buffered solution, pH 7.5. Labeled with Biotin.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
References
1. Li, et al., Physiol. Genomics 27: 42 (2006). 2. Jelinsky, et al., Proc. Natl. Acad. Sci. USA 96: 1486 (1999). 3. Kenrick, et al., A homogeneous method to quantify mRNA levels: a hybridization of Rnase protection and scintillation proximity assay technologies. Oxford University Press: 2947 (1995).
USBio References
No references available
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