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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified B IP IC FC Gel

Involved in ubiquitination of cyclin E and, in complex with RBX1, of cyclin D1 (in vitro) thus involved in regulation of G1/S transition. Seems not be part of a SCF complex with SKP1. In complex with SPOP, involved in ubiquitination of both BMI1/PCGF4 and H2AFY.
Catalog #	C7949-20B
Applications	Suitable for use in Flow Cytometry, Western Blot, Dot Blot, Immunoprecipitation, Immunocytochemistry. Other applications not tested.
Recommended Dilution	Flow Cytometry: 0.5-2ug/ml
	Western Blot: 0.2-2ug/ml
	Immunocytochemistry: 2-100ug/ml
	Immunoprecipitation: 100-500ug/ml
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	8C90
Host	Mouse
Source	Human
Concentration	~0.1mg/ml
Form	Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.05% sodium azide.
Purity	Purified by Protein G affinity chromatography.
Immunogen	Partial recombinant sequence corresponding to human Cullin 3
Specificity	Recognizes human Cullin 3. Species Crossreactivity: mouse, rat
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.