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You are here:Home » Antibodies » Abs to Cullin » Anti -Cullin 3 (CUL-3)

Anti -Cullin 3 (CUL-3)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP IC FC Gel
Involved in ubiquitination of cyclin E and, in complex with RBX1, of cyclin D1 (in vitro) thus involved in regulation of G1/S transition. Seems not be part of a SCF complex with SKP1. In complex with SPOP, involved in ubiquitination of both BMI1/PCGF4 and H2AFY.
Catalog #C7949-20B
ApplicationsSuitable for use in Flow Cytometry, Western Blot, Dot Blot, Immunoprecipitation, Immunocytochemistry. Other applications not tested.
Recommended DilutionFlow Cytometry: 0.5-2ug/ml
Western Blot: 0.2-2ug/ml
Immunocytochemistry: 2-100ug/ml
Immunoprecipitation: 100-500ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No8C90
HostMouse
SourceHuman
Concentration~0.1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 1% BSA, 0.05% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenPartial recombinant sequence corresponding to human Cullin 3
SpecificityRecognizes human Cullin 3. Species Crossreactivity: mouse, rat
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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