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You are here:Home » Antibodies » Antibodies-Cullin » Anti -Cullin 3 (CUL-3)

Anti -Cullin 3 (CUL-3)


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Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP IC FC
Involved in ubiquitination of cyclin E and, in complex with RBX1, of cyclin D1 (in vitro) thus involved in regulation of G1/S transition. Seems not be part of a SCF complex with SKP1. In complex with SPOP, involved in ubiquitination of both BMI1/PCGF4 and H2AFY.
Catalog #C7949-20B
ApplicationsSuitable for use in Flow Cytometry, Western Blot, Dot Blot, Immunoprecipitation, Immunocytochemistry. Other applications not tested.
Recommended DilutionFlow Cytometry: 0.5-2ug/ml
Western Blot: 0.2-2ug/ml
Immunocytochemistry: 2-100ug/ml
Immunoprecipitation: 100-500ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
Clone No8C90
FormSupplied as a liquid in PBS, pH 7.4, 1% BSA, 0.05% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenPartial recombinant sequence corresponding to human Cullin 3
SpecificityRecognizes human Cullin 3. Species Crossreactivity: mouse, rat
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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