C-type natriuretic peptide is produced in the hypothalamus, anterior pituitary, and most major endocrine glands. C-type natriuretic peptide has potent venodilatory and coronary vasodilatory effects, but minimal effects on renal function.
Applications
Suitable for use in ELISA. Other applications not tested.
Recommended Dilutions
ELISA: 1:3200 (0.313ug/ml) detects the HPLC purified standard peptide CNP-22 (human, rat, mouse, porcine) Optimal dilutions to be determined by the researcher.
Amino Acid Sequence
Gly-Leu-Ser-Lys-Gly-Cys-Phe-Gly-Leu-Lys-Leu-Asp-Arg-Ile-Gly- Ser-Met-Ser-Gly-Leu-Gly-Cys (Disulfide Bridge: Cys6-Cys22)
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O/PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Synthetic peptide corresponding to human CNP-22.
Form
Supplied as a lyophilized powder from PBS, pH 7.4. Reconstitute with 50ul of dH20, then add 150ul of sterile PBS.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human CNP-22. Species Crossreactivity: CNP-22 (rat, mouse, porcine), CNP-53 (rat, porcine). Does not react with CNP-53 aa1-29 (rat, porcine), alpha-ANP aa1-28 (human, canine, porcine, bovine), ANF (rat, rabbit, mouse), Endothelin-1 (human, rat, porcine, mouse, canine, bovine), porcine BNP-32, rat BNP-45, Substance P, or Urodilatin.