Cyclin A2

Clone Type

Monoclonal

Host

Mouse

Isotype

IgG2a

Clone Number

E23.1

Grade

Affinity Purified

Applications

Crossreactivity

Bo Hu Mo

Shipping Temp

Blue Ice

Storage Temp

-20°C

Cyclins regulate the activity of the Cyclin-dependent protein kinases. Cyclin A is involved in the regulation of the cell cycle and seems to be essiential for S phase and M phase. Studies have shown that overexpression of cyclin A can accelerate the G1 to S phase. Cyclin A is a marker for actively proliferating cells and for cells in S phase.

Applications

Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution

Western Blot: 0.5-1ug/ml Optimal dilutions to be determined by the researcher.

Storage and Stability

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunogen

Cyclin A fusion protein

Form

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

Purity

Purified by affinity chromatography.

Specificity

Recognizes Cyclin A. Species Crossreactivity: human, mouse and bovine.

References

1. Pagano M et al. 1992. EMBO J 11:961. 2. Geley S et al. 2001 J Cell Biol. 153:137. 3. Resinitzky D et al. 1995. Mol Cell Biol 15:4347. 4. Furuno N et al. 1999. J Cell Biol. 147:295.