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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E B IP IF

In eukaroytic cells, the cell cycle is mediated by the sequential, activation and inactivation of complexes containing cyclin dependent kinases (CDKs) and cyclins. A number of CDKs have been identified and the activity of these various CDK family members is regulated by multiple mechanisms including binding to specific cyclin proteins, binding to specific small molecular weight inhibitory protein, and reversible phosphorylation. Binding of different cyclin protein is required to activate the catalytically inactive CDKs and determines the substrate specificity of the kinase. On the other hand, CDK inhibitors exert their inhibitory effect by blocking the catalytic activity of the cyclin-bound CDKs. Since the concentration of the CDKs are in excess and remain fairly constant throughout the cell cycle, their activation is determined by the availability of the different cyclin proteins and inhibitory factors.
Catalog #	C8452-38
Applications	Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunoprecipitation. Other applications not tested.
Recommended Dilutions	Immunofluorescence: 5-10ug/ml detects Cyclin A in bovine, human, monkey and rat
	ELISA: 0.1-1ug/ml
	Western Blot: 1-2ug/ml detects Cyclin A in A431 cell lysate, bovine, HeLa cell lysate, human, monkey and mouse. Very sensitive for active Cyclin A protein.
	Immunoprecipitation: 2.5-5ug/ml detects Cyclin A in bovine, human, monkey and mouse
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2a,k
Clone No	3G111
Host	Mouse
Source	Bovine
Concentration	~0.5mg/ml
Form	Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
Purity	Purified by Protein A affinity chromatography.
Immunogen	Recombinant bovine Cyclin A protein
Specificity	Speci c for the 60kD cyclin A protein and can be utilized to detect human, monkey, mouse and bovine cyclin A. Crossreactivity with other cyclin family members has not been detected.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.