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You are here:Home » Antibodies » Antibodies-Cyclin » Anti -Cyclin B1 (G2/Mitotic-specific Cyclin-B1, CCNB, Ccnb1, Ccn-2, Ccnb1-rs13, Cycb, Cycb1) (BSA & Azide Free)

Anti -Cyclin B1 (G2/Mitotic-specific Cyclin-B1, CCNB, Ccnb1, Ccn-2, Ccnb1-rs13, Cycb,
Cycb1) (BSA & Azide Free)


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Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH FC
Cyclin B1 belongs to the cyclin family, Cyclin AB subfamily, and predicted molecular weight 48kD. Cyclin B1 is expressed ubiquitously in all eukaryotes and the level of cyclin B1 was minimal in G, began to rise in S phase, and is primarily cytoplasmic. Cyclin B1 moves into the nucleus just at the start of mitosis and associates with condensed chromosomes and the mitotic spindle. The level of cyclin B1 peaked at the G/M transition. Cyclin B1 is essential for the control of the cell cycle at the G2/M transition.
Catalog #C8453-10X
ApplicationsSuitable for use in Flow Cytometry, Western Blot and Immunohistochemistry. Not suitable for use in Immunoprecipatation. Other applications not tested.
Recommended DilutionWestern Blot: 1-2ug/ml for 2 hrs at RT
Immunohistochemistry (Formalin/paraffin): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 minutes followed by cooling at RT for 20 minutes.
Optimal dilutions to be determined by the researcher.
Positive ControlJurkat cells. Tonsil or breast carcinoma
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
Clone NoV152
FormSupplied as a liquid in 10mM PBS, pH 7.4. Also available with BSA and azide. See C8453-10.
PurityPurified by Protein G affinity chromatography.
ImmunogenHamster cyclin B1 protein.
SpecificityRecognizes hamster Cyclin B1 at ~62kDa.
Species CrossreactivityHuman, mouse and rat
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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