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You are here:Home » Antibodies » Antibodies-Cyclin » Anti -Cyclin D1

Anti -Cyclin D1


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Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP IC
Catalog #C8454-01C
ApplicationsSuitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1ug/ml detected cyclin D1 in RIPA lysates from mouse 3T3/L1 cells. 3T3/L1 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with C8454-01C (1ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.
Immunoprecipitation Immunoprecipitates cyclin D1 from rat-2 and overexpressing mouse 32Dc13 cells (1).
Immunocytochemistry Detects cyclin D1 in mouse cells.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
Clone No4H320
FormSupplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%.
PurityPurified by Protein G affinity chromatography.
ImmunogenGST fusion protein cleaved with Factor Xa and gel purified, corresponding to complete mouse Cyclin D1.
SpecificityRecognizes cyclin D1 as a doublet, Mr ~36kD. A non-specific protein was also detected, Mr 105kD. Species Crossreactivity: Mouse and rat. Does not react with human.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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