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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified B IP IC

Catalog #	C8454-01C
Applications	Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested.
Recommended Dilution	Western Blot: 1ug/ml detected cyclin D1 in RIPA lysates from mouse 3T3/L1 cells. 3T3/L1 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with C8454-01C (1ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.
Immunoprecipitation	Immunoprecipitates cyclin D1 from rat-2 and overexpressing mouse 32Dc13 cells (1).
Immunocytochemistry	Detects cyclin D1 in mouse cells.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	4H320
Host	Mouse
Source	Mouse
Concentration	~0.25mg/ml
Form	Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%.
Purity	Purified by Protein G affinity chromatography.
Immunogen	GST fusion protein cleaved with Factor Xa and gel purified, corresponding to complete mouse Cyclin D1.
Specificity	Recognizes cyclin D1 as a doublet, Mr ~36kD. A non-specific protein was also detected, Mr 105kD. Species Crossreactivity: Mouse and rat. Does not react with human.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.