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You are here:Home » Antibodies » Abs to Cyclin » Anti -Cyclin D1 (PRAD-1, bcl 1)

Anti -Cyclin D1 (PRAD-1, bcl 1)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP IH FC IF
Cyclins are regulatory subunits of the cyclin-dependent kinases (cdk), which control transition at different specific phases of the cell cycle. In mammalian cells, cyclin D1 plays a critical role in G1 to S transition. Cyclin D1 is a putative proto-oncogene overexpressed in a wide variety of human neoplasms.
Catalog #C8454-02A
ApplicationsSuitable for use in Western Blot, Immunofluorescence, Immunoprecipitation, Immunohistochemistry and Flow Cytometry. Other applications not tested.
Recommended DilutionsImmunohistochemistry: 1:50-1:100 on frozen, formalin fixed or methacarn-fixed paraffin embedded sections. Staining of formalin-fixed paraffin-embedded tissue sections requires boiling in 10mM citrate buffer, pH 6.0 for 10-20 minutes followed by cooling at RT for 20 min prior to addition of antibody.
Immunoprecipitation (Non-denatured and denatured): 10ul/mg protein lysate
Flow Cytometry: 0.25ug stains 10e6 human PBMCs fixed in 100% methanol
Western Blot: 1:250-1:500
Optimal dilutions to be determined by the researcher.
HybridomaNS2 myeloma cells with spleen cells from Balb/c mice.
Positive ControlT47D, ZR75, BT474, SKBR3, MCF-7, MDA-MB-453, U-2-OS, HT29, Ramos, Jurkat, KNRK, C32 or A431. About 50-70% of mantle cell lymphomas and 40% of greast carcinomas are positive.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG2a,k
Clone No5J223
HostMouse
SourceHuman
Concentration~0.2mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenHuman recombinant full length cyclin D1 protein.
SpecificityRecognizes human Cyclin D1at ~36kD. Species Crossreactivity: monkey, mouse, rat and canine cyclin D1.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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