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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified B IP IH FC IF

Cyclins are regulatory subunits of the cyclin-dependent kinases (cdk), which control transition at different specific phases of the cell cycle. In mammalian cells, cyclin D1 plays a critical role in G1 to S transition. Cyclin D1 is a putative proto-oncogene overexpressed in a wide variety of human neoplasms.
Catalog #	C8454-02A
Applications	Suitable for use in Western Blot, Immunofluorescence, Immunoprecipitation, Immunohistochemistry and Flow Cytometry. Other applications not tested.
Recommended Dilutions	Immunohistochemistry: 1:50-1:100 on frozen, formalin fixed or methacarn-fixed paraffin embedded sections. Staining of formalin-fixed paraffin-embedded tissue sections requires boiling in 10mM citrate buffer, pH 6.0 for 10-20 minutes followed by cooling at RT for 20 min prior to addition of antibody.
	Immunoprecipitation (Non-denatured and denatured): 10ul/mg protein lysate
	Flow Cytometry: 0.25ug stains 10e6 human PBMCs fixed in 100% methanol
	Western Blot: 1:250-1:500
	Optimal dilutions to be determined by the researcher.
Hybridoma	NS2 myeloma cells with spleen cells from Balb/c mice.
Positive Control	T47D, ZR75, BT474, SKBR3, MCF-7, MDA-MB-453, U-2-OS, HT29, Ramos, Jurkat, KNRK, C32 or A431. About 50-70% of mantle cell lymphomas and 40% of greast carcinomas are positive.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone Type	Monoclonal
Isotype	IgG2a,k
Clone No	5J223
Host	Mouse
Source	Human
Concentration	~0.2mg/ml
Form	Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide.
Purity	Purified by Protein A affinity chromatography.
Immunogen	Human recombinant full length cyclin D1 protein.
Specificity	Recognizes human Cyclin D1at ~36kD. Species Crossreactivity: monkey, mouse, rat and canine cyclin D1.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.