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C8454-02A Mouse Anti-Cyclin D1

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Source
Human
Swiss Prot
P24385
Isotype
IgG2a
Clone Number
DCS-6
Grade
Affinity Purified
Applications
FC IC IHC IP WB
Crossreactivity
Ca Hu Mk Mo Rt
Gene ID
595
Shipping Temp
Blue Ice
Storage Temp
-20°C

Cyclin D1 (PRAD1, Bcl-1) is a cytoplasmic and nuclear protein, which is synthesized during G1 phase and assembles with either cyclin-dependent kinase 4 (CDK4) or CDK6 in response to growth factor stimulation. D-type cyclin-CDK complexes act to inactivate the growth-suppressive function of the Rb protein through its phosphorylation, and titrate CDK inhibitors such as p21Cip1 and p27Kip1. Whereas during G1 phase cyclin D1 accumulates in the nucleus, it translocates into the cytoplasm during S phase. Without growth factor-mediated stimulation, cyclin D1 is unstable, and undergoes ubiquitin-mediated degradation, which is triggered by its phosphorylation. Cyclin D1 destabilization participates in G1/S phase arrest.

Applications
Suitable for use in Western Blot, Immunocytochemistry, Immunoprecipitation, Immunohistochemistry and Flow Cytometry. Other applications not tested.
Recommended Dilutions
Immunohistochemistry (frozen and paraffin): 5-10ug/ml Flow Cytometry: 3-12ug/ml. Intracellular staining. Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Full-length recombinant protein corresponding to aa1-295 from human cyclin D1.
Form
Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human Cyclin D1at ~36kD. Species Crossreactivity: monkey, mouse and rat. Does not react with feline or sheep
References
1. Bartkova, J., et al. (1996) The p16-cyclin D/Cdk4-pRb pathway as a functional unit frequently altered in melanoma pathogenesis. Cancer Research 56:5475-5483. Bartkova, J., et al. (1995) Cyclin D1 oncoprotein aberrantly accumulates in malignancies of diverse histogenesis. Oncogene 10:775-778. 2. Bartek, J., et al. (1996) Abundance and subcellular localization of cyclin D3 in human tumours. Int. J. Cancer 65:323-327. 3. De Boer, C.J., et al. (1995) Cyclin D1 protein analysis in the diagnosis of mantle cell lymphoma. Blood 86(7):2715-2723. 4. Depoortere, F., et al. (1998) A requirement for cyclin D3-cyclin-dependent kinase cdk)-4 assembly in the cyclic adenosine monophosphate-dependent proliferation of thyrocytes. The Journal of Cell Biology 140(6):1427-1439. 5. Jadayel, D.M., et al. (1997) Potential role for concurrent abnormalities of the cyclin D1, p16CDKN2 and p15CDKN2B genes in certain B cell non-Hodgkin’s lymphomas. Functional studies in a cell line (Granta 519). Leukemia 11:64-72. 6. Lukas, J., et al. (1996) Deregulated expression of E2F family members induces S-phase entry and overcomes p16INK4A-mediated growth suppression. Molecular and Cellular Biology 16(3):1047-1057.7. Lukas, J., et al. (1994) Cyclin D1 protein oscillates and is essential for cell cycle progression in human tumour cell lines. Oncogene 9:707-718. 8. Niculescu III, A., et al. (1998) Effects of p21Cip1/Waf1 at both the G1/S and the G2/M cell cycle transitions: pRb is a critical determinant in blocking DNA replication and in preventing endoreduplication. Molecular and Cellular Biology 18(1):629-643. 9. Ott, M.M., et al. (1997) Cyclin D1 expression in mantle cell lymphoma is accompanied by downregulation of cyclin D3 and is not related to the proliferative activity. Blood 90(8):3154-3159. 10. Pokrovskaja, K., et al. (1996) Phenotype-related differences in the expression of D-type cyclins in human B cell-derived lines. Cell Growth and Differentiation 7:1723-1732. 11. Steiner, P., et al. (1995) Identification of a Myc-dependent step during the formation of active G1 cyclin-cdk complexes. The EMBO Journal 14(19):4814-4826.
USBio References
No references available
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