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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified IP

Cyclin D1or PRAD-1 or bcl-1 is one of the key cell cycle regulators, and functions in association with cdk4 and/or cdk6 by phosphorylating the Rb protein. It is a putative protooncogene overexpressed in a wide variety of human neoplasms including mantle cell lymphomas (MCL)
Catalog #	C8454-05M
MW of Antigen	36kD
Epitope	Not determined
Applications and Recommended Dilutions	Immunoprecipitation (Co-precipitates Cdk4) (Native only) (Use Protein A) (Ab at 2ug/mg protein lysate)
	Kinase Assay The optimal dilution for a specific application should be determined by the investigator.
Positive Control	MAD109 cells.
Cellular Localization	Nuclear
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at-20°C or colder. Aliquots are stable for at least 12 months at-20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2a
Clone No	3F137
Host	Mouse
Source	Human
Concentration	~0.2mg/ml
Form	Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See C8454-05MX.
Purity	Purified by Protein A affinity chromatography.
Immunogen	Human recombinant full length cyclin D1 protein
Specificity	Highly specific to cyclin D1 and shows no crossreactivity with cyclin D2 or D3. It supports the cdks-associated kinase activity Species Crossreactivity: human, mouse, rat.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.