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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified B FC IF

Cyclin D2 is a G1 cyclin required for G1-phase progression and is a strong candidate for a proto-oncogene. cyclin D2 can phosphorylate pRB when associated with cdk4 and/or cdk6.
Catalog #	C8454-14
MW of Antigen	34kD
Epitope	Not determined
Applications	Flow Cytometry1
	Immunofluorescence 4
	Kinase Assay
	Western Blotting1
	Immunohistochemistry (Not suitable) The optimal dilution for a specific application should be determined by the investigator.
Positive Control	U-2-OS, C82, RD, Bristol-8, or LoVo36 cells. Note that T47D, ZR75, SKBR3, MCF-7, BT-20, MDA468, HBL100, BT5549, HeLa, Saos2, and HT29 cells are negative for cyclin D2.1
Cellular Localization	Nuclear
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at-20°C or colder. Aliquots are stable for at least 12 months at-20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2a+IgG2b
Clone No	3F138
Host	Mouse
Source	Human
Concentration	~0.2mg/ml
Form	Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See C8454-14X
Purity	Purified by Protein A affinity chromatography.
Immunogen	Puri ed histidine-tagged human recombinant full length cyclin D2 protein
Specificity	Particularly designed for sensitive detection of cyclin D2. Species Crossreactivity: human, mouse, rat.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.