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You are here:Home » Antibodies » Antibodies-Cyclin » Anti -Cyclin D2

Anti -Cyclin D2


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Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B FC IF
Cyclin D2 is a G1 cyclin required for G1-phase progression and is a strong candidate for a proto-oncogene. cyclin D2 can phosphorylate pRB when associated with cdk4 and/or cdk6.
Catalog #C8454-14
MW of Antigen 34kD
Epitope Not determined
ApplicationsFlow Cytometry1
Immunofluorescence 4
Kinase Assay
Western Blotting1
Immunohistochemistry (Not suitable) The optimal dilution for a specific application should be determined by the investigator.
Positive Control U-2-OS, C82, RD, Bristol-8, or LoVo36 cells. Note that T47D, ZR75, SKBR3, MCF-7, BT-20, MDA468, HBL100, BT5549, HeLa, Saos2, and HT29 cells are negative for cyclin D2.1
Cellular Localization Nuclear
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at-20°C or colder. Aliquots are stable for at least 12 months at-20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
Clone No3F138
FormSupplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See C8454-14X
PurityPurified by Protein A affinity chromatography.
ImmunogenPuri ed histidine-tagged human recombinant full length cyclin D2 protein
SpecificityParticularly designed for sensitive detection of cyclin D2. Species Crossreactivity: human, mouse, rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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