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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified B

Cyclin E1 and cyclin E2 can associate with and activate cdk2 (1). Upon DNA damage, upregulation/ activation of the cdk inhibitors p21Waf1/Cip1 and p27Kip1 prevents cyclin E/cdk2 activation, resulting in G1/S arrest. However, when conditions are favorable for cell cycle progression, cyclin D/cdk4/6 phosphorylates Rb and is thought to reduce the activity of p21Waf1/Cip1 and p27Kip1, allowing subsequent activation of cyclin E/cdk2 (1,2). Cyclin E/cdk2 then further phosphorylates Rb to allow progression into S-phase, where cyclin
E/cdk2 is thought to phosphorylate and activate multiple proteins involved in DNA synthesis (2,3). Turnover of cyclin E is largely contolled by phosphorylation resulting in SCFFbw7-mediated ubiquitination and proteasomedependent degradation (4,5). Cyclin E1 is phosphorylated
at multiple sites in vivo including Thr62, Ser88, Ser72, Thr380 and Ser384, and is controlled by at least two kinases, Cdk2 and GSK3 (6,7).
Catalog #	C8455-32
Applications	Suitable for use in Western Blot. Other applications have not been tested.
Recommended Dilution	Western Blot: 1:1000
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot. Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Host	Rabbit
Source	Human
Concentration	As reported
Form	Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM NaCl, 100ug/ml BSA, 50% glycerol.
Purity	Protein A and peptide affinity chromatography.
Immunogen	Synthetic peptide (KLH-coupled) corresponding to residues near the carboxy-terminus of human cyclin E2.
Specificity	Detects endogenous levels of human total cyclin E2 protein.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.