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You are here:Home » Antibodies » Antibodies-Protease Inhibitors » Anti -Cystatin D (Cystatin 10, Cst10, Carminerin)

Anti -Cystatin D (Cystatin 10, Cst10, Carminerin)


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Clone Host Grade Applications
Polyclonal Sheep Affinity Purified B
Cystatin D is a 17kD member of the type 2 cystatin family of proteins. It is expressed in salivary gland, skin, prehypertrophic and hypertrophic chondrocytes. Within the context of endochondral ossification, it may both induce chondrocyte apoptosis and initiate the process of cartilage calcification. Although Cystatin D contains a key cystatin motif (Gln-x-Val-x-Gly), Cystatin D does not appear to inhibit cysteine proteases. Mouse Cystatin D is 148aa in length. It contains one cystatin-like domain aa39-145. There are two potential splice variants. One shows a deletion of aa84-121, while a second shows a Val substitution for aa122-148. Full-length mouse Cystatin D shares 78aa identity with rat Cystatin D. There appears to be no direct human counterpart to mouse Cystatin D, as both human cystatin-C and -D share less that 40% aa identity with mouse Cystatin D.
Catalog #C8950-12
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 2ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
ConcentrationNot determined
FormSupplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 200ug/ml.
PurityPurified by immunoaffinity chromatography.
ImmunogenRecombinant corresponding to aa30-148 from Cystatin D expressed in NSO cells (NP_067380).
SpecificityRecognizes mouse Cystatin D. Species sequence homology: Recombinant human, Cst1, rhCst2, and rhCst3 (4%).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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