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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified E B

Cytochrome P450 enzymes, a superfamilyof b-type heme-containing proteins found in organisms from alldomains of life, are major catalysts in the oxidative transformationof a diversity of endogenous and exogenous compounds. CYP enzymes play an important role in the metabolic activation of environmental procarcinogens or chemical carcinogenesis, these enzymes are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The enzyme localizes to the endoplasmic reticulum and metabolizes procarcinogens such as polycyclic aromatic hydrocarbons and 17beta-estradiol. Mutations in this gene have been associated with primary congenital glaucoma; therefore it is thought that the enzyme also metabolizes a signaling molecule involved in eye development, possibly a steroid. CYP26A1 is a 497aa enzyme in mouse, rat and human (chr 10q23) and a distinct composite retinoic acid response element underlies the complex regulation of retinoic acid metabolism. This endoplasmic reticulum protein acts on retinoids, including all-trans-retinoic acid (RA), with both 4-hydroxylation and 18-hydroxylation activities. This enzyme regulates the cellular level of retinoic acid which is involved in the regulation of gene expression in both embryonic and adult tissues. Highest levels of expression are noticed in adult liver, heart, pituitary gland, adrenal gland, placenta and regions of the brain.
Catalog #	C9095-29D
Applications	Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution	ELISA: 1:100,000; using 50-100ng control peptide/well. See C9095-29E.
	Western Blot: 1-10ug/ml using Chemiluminescence
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Human
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, pH 7.2, 0.1% BSA.
Purity	Purified by immunoaffinity chromatography.
Immunogen	Synthetic peptide corresponding to 18aa from the C-terminus of human CYP26A1 (KLH). Species Sequence Homology: mouse (100%), chicken (55%).
Specificity	Recognizes human Cytochrome P450 26A1 (CYP26A1).
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.