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You are here:Home » Antibodies » Abs to Cytokeratin » Anti -Cytokeratin

Anti -Cytokeratin

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH
Catalog #C9097-01B
Recommended Secondary Reagents F(ab')2 rabbit anti-mouse IgG HRP
Fusion Partners Spleen cells from immunized BALB/c mice were fused with cells of the NS1/Ag4.1 myeloma cell line.
Recommended Positive Control Tissue Human kidney
Recommended Negative Controls Mouse IgG1 Negative Control
ApplicationsWestern Blot: Reported
Immunohistochemistry: (frozen)- 1:50, (paraffin)- 1:50
Optimal working dilutions to be determined by researcher.
Immunohistochemistry NB This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections. However if using a ventana autostainer, heat mediated pre-treatment with EDTA pH 8.0 is recommended.
Clone TypeMonoclonal
IsotypeIgG1
Clone No3H2049
HostMouse
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in PBS, 0.2% BSA, 0.1% sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenHuman keratin isolated from epidermal stratum corneum.
SpecificityReacts with cytokeratin polypeptides No 1, 2, 5, 6, 7, 8, 11, 14, 16, 17 and 18. In tissue sections it binds to cells of epithelial origin; non-epithelial cells and skin based layer cells are negative. It has also been shown to stain neoplastic cells of epithelial origin. Clone 3H2049 has been reported as being suitable for use in Western blotting. The molecular weight of the recognized antigen is 56kD. Species Crossreactivity: Japanese Macaque (4), cynomolgus monkey (5).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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