| Catalog # | C9097-01B |
| Recommended Secondary Reagents | F(ab')2 rabbit anti-mouse IgG HRP |
| Fusion Partners | Spleen cells from immunized BALB/c mice were fused with cells of the NS1/Ag4.1 myeloma cell line. |
| Recommended Positive Control Tissue | Human kidney |
| Recommended Negative Controls | Mouse IgG1 Negative Control |
| Applications | Western Blot: Reported |
| Immunohistochemistry: (frozen)- 1:50, (paraffin)- 1:50 |
| Optimal working dilutions to be determined by researcher. |
| Immunohistochemistry | NB This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections. However if using a ventana autostainer, heat mediated pre-treatment with EDTA pH 8.0 is recommended. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG1 |
| Clone No | 3H2049 |
| Host | Mouse |
| Source | Human |
| Concentration | As reported |
| Form | Supplied as a liquid in PBS, 0.2% BSA, 0.1% sodium azide. |
| Purity | Purified by Protein A affinity chromatography. |
| Immunogen | Human keratin isolated from epidermal stratum corneum. |
| Specificity | Reacts with cytokeratin polypeptides No 1, 2, 5, 6, 7, 8, 11, 14, 16, 17 and 18. In tissue sections it binds to cells of epithelial origin; non-epithelial cells and skin based layer cells are negative. It has also been shown to stain neoplastic cells of epithelial origin. Clone 3H2049 has been reported as being suitable for use in Western blotting. The molecular weight of the recognized antigen is 56kD. Species Crossreactivity: Japanese Macaque (4), cynomolgus monkey (5). |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
