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You are here:Home » Antibodies » Antibodies-Cytokeratin » Anti -Cytokeratin 14

Anti -Cytokeratin 14


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Clone Host Grade Applications
Monoclonal Mouse Highly Purified B IH FC IF
Cytokeratin 14 is one of two cytokeratins that can distinguish stratifying epithelial cell types from simple epithelial cell types, being expressed by stratifying/keratinocytes cell types but not by simple epithelial cells.
Catalog #C9097-13A
ApplicationsSuitable for use in Flow Cytometry, Immunohistochemistry, Western Blot and Immunofluorescence. Other applications not tested.
Recommended DilutionsFlow Cytometry: 1:100. Use10ul to label 10e6 cells in 100ul. Membrane permeabilization is required.
Immunohistochemistry: frozen sections
Immunohistochemistry (paraffin): 1:200. Requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer is recommended for this purpose.
Optimal dilutions to be determined by the researcher.
Recommended Secondary AntibodiesI1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
I1904-14C: IgG, Fc (HRP) Pab Gt xMo
I1904-19C: IgG, F(abí)2 (HRP) Pab Gt xMo
Recommended Positive Control TissueSkin
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
Clone No3H2053
FormSupplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
PurityPurified by Ion Exchange chromatography.
ImmunogenLast 15 C-terminal residues of human cytokeratin 14 conjugated to thyroglobulin.
SpecificityRecognizes human cytokeratin 14, a type I intermediate filament. Species Crossreactivity: elephant, canine, porcine
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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