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Specifications

Clone Host Grade Applications Monoclonal Mouse Highly Purified B IH FC IF

Cytokeratin 14 is one of two cytokeratins that can distinguish stratifying epithelial cell types from simple epithelial cell types, being expressed by stratifying/keratinocytes cell types but not by simple epithelial cells.
Catalog #	C9097-13A
Applications	Suitable for use in Flow Cytometry, Immunohistochemistry, Western Blot and Immunofluorescence. Other applications not tested.
Recommended Dilutions	Flow Cytometry: 1:100. Use10ul to label 10e6 cells in 100ul. Membrane permeabilization is required.
	Immunohistochemistry: frozen sections
	Immunohistochemistry (paraffin): 1:200. Requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer is recommended for this purpose.
	Optimal dilutions to be determined by the researcher.
Recommended Secondary Antibodies	I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
	I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
	I1904-14C: IgG, Fc (HRP) Pab Gt xMo
	I1904-19C: IgG, F(ab’)2 (HRP) Pab Gt xMo
Recommended Positive Control Tissue	Skin
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone Type	Monoclonal
Isotype	IgG3
Clone No	3H2053
Host	Mouse
Source	Human
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Purity	Purified by Ion Exchange chromatography.
Immunogen	Last 15 C-terminal residues of human cytokeratin 14 conjugated to thyroglobulin.
Specificity	Recognizes human cytokeratin 14, a type I intermediate filament. Species Crossreactivity: elephant, canine, porcine
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.