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You are here:Home » Antibodies » Abs to Cytokeratin » Anti -Cytokeratin 15

Anti -Cytokeratin 15

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Specifications

Clone Host Grade Applications
Polyclonal Guinea pig Serum B IH
Cytokeratin 15 is a 50kD (pI 4.9) intermediate filament polypeptide detected by immunofluorescence microscopy in basal cells of stratified epithelia (including epidermis, tongue, esophagus, exocervix) and complex stratified epithelia (e.g. broncheal epithelium), in luminal epithelia of mammary, salivary and sweat gland. In the hair follicle, staining was restricted to the outermost cell layer of the outer root sheath; within the hair follicle several cell layers of the hair follicle bulb were positive with this antibody. In human fetal tissues cytokeratin 15 was localized in basal cells of epidermis, lung, trachea, tracheal glands and esophagus. In fetal hair anlagen, all cells were positive with this antibody, suggesting that cytokeratin 15 is preferentially expressed in epithelial stem cells.
Catalog #C9097-15A
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications have not been tested.
Recommended DilutionWestern Blot: 1:2000 (ECL)
Immunohistochemistry (frozen sections): 1:100-1:200; Incubation time-1 hr at RT.
Optimal dilutions to be determined by the researcher.
Reactivity on Cultured Cell LinesPositive on HaCat and A-43 and negative on PLC.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGuinea pig
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid with 0.09% sodium azide.
PuritySerum
ImmunogenC-terminal tail region (including parts of the rod domain) of human recombinant cytokeratin 15.
SpecificityIn tumors, this antibody is reactive with squamous skin and lung carcinomas and with a significant subgroup of invasive ductal breast carcinomas, especially tumors with higher grade malignancy (Heid, Bartek, Leube, Moll, Kaufmann & Franke). Completely unreactive were cells of liver, colon, kidney, urinary bladder, pancreas and lung alveolus. Species Crossreactivity: Human and mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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