Suitable for use in Flow Cytometry, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Immunohistochemistry (Frozen, Paraffin-embedded): 1:10; 1hr at 37°C. Optimal dilutions to be determined by the researcher.

Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Human cytokeratin 18 from HeLa cytoskeletal preparation.

Supplied as a liquid, 0.09% sodium azide. Labeled with Fluorescein isothiocyanate (FITC).

Purified by Protein A affinity chromatography.

Recognizes Cytokeratin 18 (MW: 45kD) of all human simple type epithelia and basal cells of many squamous, nonepidermal epithelia. Reacts with cultured HeLa and MCF-7 cells. |Species Crossreactivity: mouse, rat, bovine, porcine, canine, hamster, sheep and fish (trout).