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C9097-28C Mouse Anti-Cytokeratin 19

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Source
Human
Swiss Prot
P08727
Isotype
IgG1,k
Clone Number
RCK108
Grade
Purified
Applications
FC IC IHC WB
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C

Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40kD and 68kD and isoelectric pH between 4.9-7.8. The individual cytokeratin polypeptides are designated 1 to 20. Cytokeratin 1 has the highest molecular weight and the highest isoelectric pH, while cytokeratin 19 has the lowest molecular weight and a low isoelectric pH. The cytokeratins are divided into the type I and type II subgroups, the type II family members comprising the basic to neutral cytokeratins 1-8, while the type I group comprises the acidic cytokeratins 9-20. The cytokeratins are expressed in combinations which characterize the type of epithelium. Cytokeratin 19 is the smallest human cytokeratin (40kD) and is found in many simple and invariably in some non-keratinizing stratified squamous epithelia. All adenocarcinomas tested have been positive for cytokeratin 19.

Applications
Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry and Immunocytochemistry. Other applications not tested.
Recommended Dilutions
Immunohistochemistry: 1:100-1:200 on frozen and paraffin embedded tissue (after treatment with pepsin or trypsin) sections. Western Blot: 1:100-1:1000; Recognizes a 40kD band in reduced westerns Immunocytochemistry: 1:100-1:200 Flow Cytometry: 1:100-1:200 Optimal dilution determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Cytoskeletal preparation of T24 cells
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity
Purified from culture supernatant.
Specificity
Recognizes human Cytokeratin 19. Reacts with glandular epithelial cells and most carcinomas. Does not react with hepatocytes and hepatocarcinoma.
References
1. Kwaspen,FH. et al., Reproducible and highly sensitive detection of the broad spectrum epithelial marker keratin 19 in routine cancer diagnosis. Histopathology (1997) 31:503–516. 2. Schaafsma, HE, Ramaekers FCS, Cytokeratin subtyping in normal and neoplastic epithelium: basic principles and diagnostic applications. Pathol. Ann. (1994) 29:21–62.
USBio References
No references available
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