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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified B IH

Catalog #	C9097-37K
Applications	Suitable for use in Immunohistochemistry (frozen and paraffin-embedded tissue and cytological material) and Immunoblot (Western).
Recommended Dilution	Immunohistochemistry: 1:10; Incubation Time 1h at RT, extended with paraffin sections (overnight at 2-8°C). Pretreatment with paraffin embedded sections, protease pretreatment is required prior to antibody application.
Storage and Stability	Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone Type	Monoclonal
Isotype	IgG1
Clone No	5F173
Host	Mouse
Source	Human
Concentration	~0.05mg/ml
Form	Supplied as a lyophilized powder in PBS, pH 7.4, 0.5% BSA, 0.09% sodium azide. Reconstitute to a final volume of 1ml.
Purity	Purified by Protein A affinity chromatography.
Immunogen	Human cytokeratin 8, purified from SDS PAGE.
Specificity	Reactivities on Cultured MCF-7, RT 112, HT-29, Detroit 562, RPMI 2650. Cell lines (tested so far) SSC-12, bovine BMGE+H, BMGE-H, MDBK. Represents an excellent marker for distinguishing carcinomas from all non-epithelial tumors. Specifically reacts with cytokeratins 5 and 8 present in nearly all epithelia. Polypeptide reacting
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.