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You are here:Home » Antibodies » Antibodies-Cytokeratin » Anti -Cytokeratin 5,8

Anti -Cytokeratin 5,8


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Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH
Catalog #C9097-37K
ApplicationsSuitable for use in Immunohistochemistry (frozen and paraffin-embedded tissue and cytological material) and Immunoblot (Western).
Recommended DilutionImmunohistochemistry: 1:10; Incubation Time 1h at RT, extended with paraffin sections (overnight at 2-8°C). Pretreatment with paraffin embedded sections, protease pretreatment is required prior to antibody application.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
Clone No5F173
FormSupplied as a lyophilized powder in PBS, pH 7.4, 0.5% BSA, 0.09% sodium azide. Reconstitute to a final volume of 1ml.
PurityPurified by Protein A affinity chromatography.
ImmunogenHuman cytokeratin 8, purified from SDS PAGE.
SpecificityReactivities on Cultured MCF-7, RT 112, HT-29, Detroit 562, RPMI 2650. Cell lines (tested so far) SSC-12, bovine BMGE+H, BMGE-H, MDBK. Represents an excellent marker for distinguishing carcinomas from all non-epithelial tumors. Specifically reacts with cytokeratins 5 and 8 present in nearly all epithelia. Polypeptide reacting
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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