| Catalog # | C9097-37K2 |
| Represents an excellent marker for distinguishing carcinomas from all non-epithelial tumors. Specifically reacts with cytokeratins 5 and 8 present in nearly all epithelia. Polypeptide reacting | Mr 52,500, Mr 58,000 cytokeratins (type II cytokeratins nos. 5+8) of human epithelial cells. Epitope has been mapped to aa353-367 on alpha helical rod domain. |
| Applications | Suitable for use in FACS and Immunohistochemistry. Other applications have not been tested. |
| Recommended Dilution | Immunohistochemistry (Frozen): 1:10 for 1hr at RT. |
| Immunohistochemistry (Paraffin-embedded tissue and cytological material): 1:10 at 4°C O/N. Requires protease pretreatment prior to antibody application. |
| Optimal dilutions to be determined by the researcher. |
| Positive Controls | MCF-7, RT 112, HT-29, Detroit 562, RPMI 2650, SSC-12, bovine BMGE+H, BMGE-H, MDB cell lines |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| Clone Type | Monoclonal |
| Isotype | IgG1 |
| Clone No | 5F173 |
| Host | Mouse |
| Source | Human |
| Concentration | Not determined |
| Form | Supplied as a liquid. Conjugated to FITC. |
| Purity | Purified by Protein A affinity chromatography. |
| Immunogen | Human cytokeratin 8, purified from SDS PAGE. |
| Specificity | Recognizes human Cytokeratin 5,8. Species Crossreactivity: Bovine, murine, amphibia, porcine and hydra. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
