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You are here:Home » Antibodies » Abs to Cytokeratin » Anti -Cytokeratin 5,8 (FITC)

Anti -Cytokeratin 5,8 (FITC)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified IH FC
Catalog #C9097-37K2
Represents an excellent marker for distinguishing carcinomas from all non-epithelial tumors. Specifically reacts with cytokeratins 5 and 8 present in nearly all epithelia. Polypeptide reacting Mr 52,500, Mr 58,000 cytokeratins (type II cytokeratins nos. 5+8) of human epithelial cells. Epitope has been mapped to aa353-367 on alpha helical rod domain.
ApplicationsSuitable for use in FACS and Immunohistochemistry. Other applications have not been tested.
Recommended DilutionImmunohistochemistry (Frozen): 1:10 for 1hr at RT.
Immunohistochemistry (Paraffin-embedded tissue and cytological material): 1:10 at 4°C O/N. Requires protease pretreatment prior to antibody application.
Optimal dilutions to be determined by the researcher.
Positive ControlsMCF-7, RT 112, HT-29, Detroit 562, RPMI 2650, SSC-12, bovine BMGE+H, BMGE-H, MDB cell lines
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No5F173
HostMouse
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid. Conjugated to FITC.
PurityPurified by Protein A affinity chromatography.
ImmunogenHuman cytokeratin 8, purified from SDS PAGE.
SpecificityRecognizes human Cytokeratin 5,8. Species Crossreactivity: Bovine, murine, amphibia, porcine and hydra.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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