USBio Logo

C9097-37K2 Mouse Anti-Cytokeratin 5,8 (FITC)

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Source
Human
Conjugate
FITC
Isotype
IgG1
Clone Number
5F173 (C22)
Grade
Affinity Purified
Applications
FC IHC
Crossreactivity
Bo Hu Mo Po
Shipping Temp
Blue Ice
Storage Temp
-20°C

Represents an excellent marker for distinguishing carcinomas from all non-epithelial tumors. Specifically reacts with cytokeratins 5 and 8 present in nearly all epithelia. Polypeptide reacting: Mr 52,500, Mr 58,000 cytokeratins (type II cytokeratins nos. 5+8) of human epithelial cells. Epitope has been mapped to aa353-367 on alpha helical rod domain.

Applications
Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications have not been tested.
Recommended Dilution
Immunohistochemistry (Frozen): 1:10 for 1hr at RT. Immunohistochemistry (Paraffin-embedded tissue and cytological material): 1:10 at 4°C O/N. Requires protease pretreatment prior to antibody application. Optimal dilutions to be determined by the researcher.
Positive Controls
MCF-7, RT 112, HT-29, Detroit 562, RPMI 2650, SSC-12, bovine BMGE+H, BMGE-H, MDB cell lines
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Human cytokeratin 8, purified from SDS PAGE.
Form
Supplied as a liquid. Labeled with Fluorescein isothiocyanate (FITC).
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes human Cytokeratin 5,8. Species Crossreactivity: Bovine, murine, amphibia, porcine and hydra.
References
1. Bartek, J., et al., J. Pathol. 164: 215-224 (1991). 2. Moll, R., et al., Cell 31: 11-24 (1982). 3. Waseem, A., et al., Biochemistry 43: 1283-1295 (2004).
USBio References
No references available
Conjugates
Pricing
Order
Proceed to Checkout
Cart Summary
ProductSizeListYour PriceQtyExt Price
Subtotal:Subtotal:
Subtotal:Subtotal:
Total Coupon Savings:Total Coupon Savings:()
Your cart is currently empty.
- Coupon Code
Recently Viewed
Contact Us

Visit our technical library or contact our support staff to answer your questions.

Telephone:
1-800-520-3011

Library | Contact

Distributors

For customers outside of the United States, please use one of our many distributors.

View Distributors

Payment Methods

We accept the following payment methods as well as pay-by-invoice.

MasterCard Visa PayPal
© 2023-2024 United States Biological - All Rights Reserved