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Specifications

Clone Host Grade Applications Polyclonal Guinea pig Serum B IH

CK9 can be detected in primary cultures of palmoplantar keratinocytes when they shift to differentiation-promoting conditions and grow stratified (upper cells). CK9 has not been found in normal, i.e. non-pathogenic, non-ridged epidermis, beside some minor cells surrounding the acrosyringeal ducts. No labelling has been found in epithelial cells of other stratified epithelia such as oe-sophagus or complex epithelia (e.g. urothelium) or in ductal or simple epithelia. Negative tissues include: muscle, liver and duodenum. (see references listed below).
Catalog #	C9097-43
Applications	Suitable for use in Western Blot and Immunohistochemistry (frozen and paraffin-embedded cells and tissue).
Recommended Dilution	Immunohistochemistry: 1:100-1:200; 1:50 for paraffin-embedded tissue; Incubation Time 1 hr at RT; extended with paraffin.
	Western Blot: 1:5000-1:10,000 (ECL)
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone Type	Polyclonal
Isotype	IgG
Host	Guinea pig
Concentration	Not determined
Form	Supplied as a liquid, 0.5% BSA and 0.09% sodum azide.
Purity	Serum
Immunogen	Synthetic peptide of human cytokeratin 9 (corresponding to aa. 450-477), coupled to KLH.
Specificity	Reactive Polypeptide: Acidic human cytokeratin 9 (MW 62,129), expressed in the palmoplantar epidermis. The antiserum represents an excellent marker to study palmoplantar epidermal distribution and differentiation, specifically reactive in the middle/upper suprabasal layers (stratum spinosum/granulosum) of the epidermis of palm and sole.
Species Crossreactivity	Human, bovine, mouse and rat.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.