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Specifications

Clone Host Grade Applications Monoclonal Mouse Purified IH

Cytokeratins are a family of water-insoluble intermediate laments, composed of polypeptide chains found in the cytoskeleton of epithelial cells. Twenty different forms of human cytokeratins exist (Moll numbers 1–20) in both normal and malignant epithelial cell lines. (1) Speci c combinations of cytokeratin peptides are associated with certain epithelial cells, and are therefore useful in the characterization of poorly differentiated carcinoma. (2–4) Cytokeratins may also be clinically signi cant indicators in distinguishing carcinoma from malignant tumors of non-epithelial origin, such as lymphoma, sarcoma, and melanoma. Cytokeratin 8 and 18 staining has also been observed in renal cell carcinomas, hepatocellular carcinomas, cholangiocarcinomas, germ cell tumors and some neuroendocrine carcinomas, epithelioid sarcomas and non-epithelial tumors. (7)
Catalog #	C9097-44B
Applications	Suitable for use in Immunohistochemistry. Other applications not tested.
Recommended Dilution	Immunohistochemistry: Stains cytokeratins 8 and 18 in frozen tissue or formalin-fixed, paraffin-embedded (FFPE) tissue sections.
	Optimal dilutions to be determined by the researcher.
Epitope retrieval	Required (Citrate buffer, pH 6.0).
Enzyme digestion	Required.
Control Tissue	Normal colon
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone Type	Monoclonal
Isotype	IgG1
Clone No	3G126
Host	Mouse
Source	Human
Concentration	~0.5mg/ml
Form	Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.1% sodium azide.
Purity	Purified
Immunogen	Human keratin 8 & 18. Expected Staining Pattern: Cytoplasmic and membrane
Specificity	Recognizes human cytokeratins with molecular weights 52 and 45kD, corresponding to cytokeratins 8 and 18, respectively. Useful in the detection of adenocarcinomas, mesotheliomas and certain squamous epithelia-derived carcinomas. Interestingly, some epithelial tumors demonstrate cytokeratin 8 and 18 expression, but normal strati ed squamous epithelium does not.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.