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C9097-44B Mouse Anti-Cytokeratin 8,18

Specifications
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG1
Clone Number
3G126
Grade
Purified
Applications
IHC
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C

Cytokeratins are a family of water-insoluble intermediate filaments, composed of polypeptide chains found in the cytoskeleton of epithelial cells. Twenty different forms of human cytokeratins exist (Moll numbers 1–20) in both normal and malignant epithelial cell lines. (1) Specific combinations of cytokeratin peptides are associated with certain epithelial cells, and are therefore useful in the characterization of poorly differentiated carcinoma. (2–4) Cytokeratins may also be clinically significant indicators in distinguishing carcinoma from malignant tumors of non-epithelial origin, such as lymphoma, sarcoma, and melanoma. Cytokeratin 8 and 18 staining has also been observed in renal cell carcinomas, hepatocellular carcinomas, cholangiocarcinomas, germ cell tumors and some neuroendocrine carcinomas, epithelioid sarcomas and non-epithelial tumors. (7)

Applications
Suitable for use in Immunohistochemistry. Other applications not tested.
Recommended Dilution
Immunohistochemistry: Stains cytokeratins 8 and 18 in frozen tissue or formalin-fixed, paraffin-embedded (FFPE) tissue sections. Optimal dilutions to be determined by the researcher.
Epitope retrieval
Required (Citrate buffer, pH 6.0).
Enzyme digestion
Required.
Control Tissue
Normal colon
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Human keratin 8 & 18. Expected Staining Pattern: Cytoplasmic and membrane
Form
Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.09% sodium azide.
Purity
Purified
Specificity
Recognizes human cytokeratins with molecular weights 52 and 45kD, corresponding to cytokeratins 8 and 18, respectively. Useful in the detection of adenocarcinomas, mesotheliomas and certain squamous epithelia-derived carcinomas. Interestingly, some epithelial tumors demonstrate cytokeratin 8 and 18 expression, but normal stratified squamous epithelium does not.
Conjugates
Pricing
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