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Specifications

Clone Host Grade Applications Polyclonal Guinea pig Serum B IH IF

Catalog #	C9097-49
Applications	Suitable for use in Immunohistochemistry, Immunofluorescence Microscopy and Western Blot. Other applications have not been tested.
Recommended Dilutions	Immunohistochemistry (Frozen): 1:100-1:200. Incubate at RT for 1 hour.
	Western Blot: 1:2000 (ECL)
	Immunofluorescence Microscopy: 1:100-200 using acetone fixation) and 1:100 using 4% paraformaldehyde fixation.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone Type	Polyclonal
Isotype	IgG
Host	Guinea pig
Source	Human
Concentration	Not Determined
Form	Supplied as a liquid, 0.09% sodium azide.
Purity	Serum
Immunogen	Synthetic peptide corresponding to human type II cytokeratin, C-FTTSGGHSLGAGLGGSGFSATSNRGL conjugated to KLH.
Specificity	Recognizes human Cytokeratin K6hf. Stains specifically the companion layer (innermost layer of outer root sheath) of the human hair follicle. No crossreaction with cytokeratins 5 and 6, although there is considerable sequence homology. Species Crossreactivity: mouse.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.