Suitable for use in Immunohistochemistry, Immunofluorescence Microscopy and Western Blot. Other applications have not been tested.
Recommended Dilutions
Immunohistochemistry (Frozen): 1:100-1:200. Incubate at RT for 1 hour. Western Blot: 1:2000 (ECL) Immunofluorescence Microscopy: 1:100-200 using acetone fixation) and 1:100 using 4% paraformaldehyde fixation. Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to human type II cytokeratin, C-FTTSGGHSLGAGLGGSGFSATSNRGL conjugated to KLH.
Form
Supplied as a liquid, 0.5% BSA, 0.09% sodium azide.
Purity
Serum
Specificity
Recognizes human Cytokeratin K6hf. Stains specifically the companion layer (innermost layer of outer root sheath) of the human hair follicle. No crossreaction with cytokeratins 5 and 6, although there is considerable sequence homology. Species Crossreactivity: mouse.
References
1. Winter, H., et al., J. Invest. Dermatol. 111: 955-962 (1998). 2. Langbein, L., et al., Methods in Cell Biology 78: 413-456 (2004). 3. Wang z, Wong P., J Invest Dermatol 121:1276-11282 (2003).