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You are here:Home » Antibodies » Antibodies-Cytokeratin » Anti -Cytokeratin K6hf

Anti -Cytokeratin K6hf


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Clone Host Grade Applications
Polyclonal Guinea pig Serum B IH IF
Catalog #C9097-49
ApplicationsSuitable for use in Immunohistochemistry, Immunofluorescence Microscopy and Western Blot. Other applications have not been tested.
Recommended DilutionsImmunohistochemistry (Frozen): 1:100-1:200. Incubate at RT for 1 hour.
Western Blot: 1:2000 (ECL)
Immunofluorescence Microscopy: 1:100-200 using acetone fixation) and 1:100 using 4% paraformaldehyde fixation.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
HostGuinea pig
ConcentrationNot Determined
FormSupplied as a liquid, 0.09% sodium azide.
ImmunogenSynthetic peptide corresponding to human type II cytokeratin, C-FTTSGGHSLGAGLGGSGFSATSNRGL conjugated to KLH.
SpecificityRecognizes human Cytokeratin K6hf. Stains specifically the companion layer (innermost layer of outer root sheath) of the human hair follicle. No crossreaction with cytokeratins 5 and 6, although there is considerable sequence homology. Species Crossreactivity: mouse.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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