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D3224-30 Rabbit Anti-DFF40 (CAD, DNA Fragmentation Factor, Caspase Activated Deoxyribonuclease, CPAN)

Specifications
References
Clone Type
Polyclonal
Host
Rabbit
Source
Mouse
Isotype
IgG
Grade
Affinity Purified
Applications
IC IF WB
Crossreactivity
Hu Mo Rt
Shipping Temp
Blue Ice
Storage Temp
-20°C

Apoptosis, the process of programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms and involves dramatic changes in cellular structure. Disruption of apoptosis may contribute to cancer and to autoimmune and degenerative diseases. Apoptosis is induced by a family of cell death receptors and their corresponding ligands. Cell death signals are then transduced by adapter molecules and members of the caspase (ICE/CED-3) family of cysteine proteases that eventually lead to the degradation of chromosomal DNA by activated DNase. Recently identified was a mouse DNase that causes apoptotic DNA fragmentation and was designated CAD (for caspase activated deoxyribonuclease) (1,2). The human homologue of mouse CAD was recently identified by two groups independently and termed CPAN (caspase activated nuclease) and DFF40 (DNA fragmentation fragment 40) (3,4). Human DFF45 and its mouse homologue ICAD are the inhibitors of DFF40 and CAD, respectively (5). DFF is composed of two subunits, DFF40/CAD and DFF45/ICAD. Cleavage of DFF45/ICAD, which is mediated by caspase-3, leads to DFF40/CADís activation as a nuclease (1–4). Activation of DFF40/CAD, which causes DNA degradation, is the hallmark of apoptotic cell death. DFF40 mRNA is expressed in limited number of human tissues; pancreas, spleen, prostate, and ovary (6).

Applications
Suitable for use in Western Blot, Immunofluorescence and Immunocytochemistry. Other applications not tested.
Recommended Dilutions
Western Blot: 0.5ug/ml Immunocytochemistry: 5ug/ml Optimal dilutions to be determined by researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to the sequence near the middle of mouse DFF40/CAD. Species sequence homology: bovine
Form
Supplied as a liquid in PBS, 0.02% sodium azide.
Purity
Purified by peptide affinity chromatography.
Specificity
Recognizes mouse DFF40/CAD. Species Crossreactivity: human and rat
References
1. Enari M, Sakahira H, Yokoyama H, Okawa K, Iwamatsu A, Nagata S. A caspase-activated DNase that degrades DNA during apoptosis, and its inhibitor ICAD. Nature 1998;391:43-50. 2. Sakahira H, Enari M, Nagata S. Cleavage of CAD inhibitor in CAD activation and DNA degradation during apoptosis. Nature 1998;391:96-99. 3. Halenbeck R, MacDonald H, Roulston A, Chen TT, Conroy L, Williams LT. CPAN, a human nuclease regulated by the caspase-sensitive inhibitor DFF45. Curr Biol 1998;8:537-40. 4. Liu X, Li P, Widlak P, Zou H, Luo X, Garrard WT, Wang X The 40-kDa subunit of DNA fragmentation factor induces DNA fragmentation and chromatin condensation during apoptosis. Proc Natl Acad Sci USA 1998;95:8461-6. 5. Mukae N, Enari M, Sakahira H, Fukuda Y, Inazawa J, Toh H, Nagata S. Molecular cloning and characterization of human caspase-activated DNase. Proc Natl Acad Sci USA 1998;95:9123-8|6. Liu X, Zou H, Slaughter C, Wang X. DFF, a heterodimeric protein that functions downstream of caspase-3 to trigger DNA fragmentation during apoptosis. Cell 1997;89:175-184 |
USBio References
No references available
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