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You are here:Home » Antibodies » Abs to Enzymes, Protease (Proteinase, Peptidase) » Anti -Dipeptidyl Peptidase II (DPPII, Quiescent cell proline dipeptidase, QPP, DPP7)

Anti -Dipeptidyl Peptidase II (DPPII, Quiescent cell proline dipeptidase, QPP, DPP7)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Rat Affinity Purified E B N
Dipeptidyl-peptidase II (DPPII), also known as quiescent cell proline dipeptidase (QPP) and
DPP7, shares some substrate and cleavage specificity with DPPIV/CD26, DPP8, DPP9 and
seprase/FAP (fibroblast activation protein), members of the S09 family of serine proteases.
They are all prolyl proteases that cleave proteins and peptides after proline residues.
However, DPPII is not a member of the S09 family, but a member of the S28 family that also
includes lysosomal Pro-X carboxypeptidase/prolylcarboxypeptidase/PRCP and
thymus-specific serine peptidase/PRSS16.
Catalog #D8065-31B
ApplicationsSuitable for use in ELISA, Neutralization and Western blot. Other applications have not been tested.
Recommended Dilutions:
Neutralization-preincubated with the
enzyme at different molar ratios at room temperature for 30 minutes. The enzyme was then
assayed with a fluorogenic peptide substrate. 50% of the proteolytic activity was inhibited by
the antibody at approximately 0.12ug/ml (IC50) under conditions in which the enzyme was
present at 0.1ug/ml and the substrate concentration was 10uM. Considering the molecular
masses of the enzyme (53kD) and the antibody (150kD), IC50 was achieved at
approximately 1:2 molar ratio of the antibody to the enzyme.
Western blot: 1-2ug/ml The detection limit is ~10 ng/lane under non-reducing conditions. Reducing conditions are not recommended. Chemiluminescent detection with
WesternGloTM Chemiluminescent Detection Substrate will increase sensitivity by 5 to 50 fold.
Optimal dilutions to be determined by the researcher.
Hybridomamouse myeloma with B cells obtained from a rat immunized with purified, NS0-derived, recombinant human DPPII.
Storage and Stability
Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No8B15
HostRat
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a lyophilized powder in PBS with 5% trehalose. Reconstitute with sterile PBS to a concentration of 0.5mg/ml.
PurityPurified by Protein G affinity chromatography.
ImmunogenNS0-derived recombinant human rhDPPII..
SpecificityRecognizes human DPPII. Species sequence homology: chimpanzee 99%, mouse 81%, rat 80%, dog 79% and chicken 65%.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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