| Dipeptidyl-peptidase II (DPPII), also known as quiescent cell proline dipeptidase (QPP) and |
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| DPP7, shares some substrate and cleavage specificity with DPPIV/CD26, DPP8, DPP9 and |
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| seprase/FAP (fibroblast activation protein), members of the S09 family of serine proteases. |
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| They are all prolyl proteases that cleave proteins and peptides after proline residues. |
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| However, DPPII is not a member of the S09 family, but a member of the S28 family that also |
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| includes lysosomal Pro-X carboxypeptidase/prolylcarboxypeptidase/PRCP and |
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| thymus-specific serine peptidase/PRSS16. |
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| Catalog # | D8065-31B |
| Applications | Suitable for use in ELISA, Neutralization and Western blot. Other applications have not been tested. |
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| Recommended Dilutions: |
| Neutralization-preincubated with the |
| enzyme at different molar ratios at room temperature for 30 minutes. The enzyme was then |
| assayed with a fluorogenic peptide substrate. 50% of the proteolytic activity was inhibited by |
| the antibody at approximately 0.12ug/ml (IC50) under conditions in which the enzyme was |
| present at 0.1ug/ml and the substrate concentration was 10uM. Considering the molecular |
| masses of the enzyme (53kD) and the antibody (150kD), IC50 was achieved at |
| approximately 1:2 molar ratio of the antibody to the enzyme. |
| Western blot: 1-2ug/ml The detection limit is ~10 ng/lane under non-reducing conditions. Reducing conditions are not recommended. Chemiluminescent detection with |
| WesternGloTM Chemiluminescent Detection Substrate will increase sensitivity by 5 to 50 fold. |
| Optimal dilutions to be determined by the researcher. |
| Hybridoma | mouse myeloma with B cells obtained from a rat immunized with purified, NS0-derived, recombinant human DPPII. |
| Storage and Stability | |
| Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| Clone Type | Monoclonal |
| Isotype | IgG2a |
| Clone No | 8B15 |
| Host | Rat |
| Source | Human |
| Concentration | ~0.5mg/ml |
| Form | Supplied as a lyophilized powder in PBS with 5% trehalose. Reconstitute with sterile PBS to a concentration of 0.5mg/ml. |
| Purity | Purified by Protein G affinity chromatography. |
| Immunogen | NS0-derived recombinant human rhDPPII.. |
| Specificity | Recognizes human DPPII. Species sequence homology: chimpanzee 99%, mouse 81%, rat 80%, dog 79% and chicken 65%. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
