Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Antibodies » Abs to G Protein Receptors » Anti -EDG1 (Endothelial Cell Differentiation Gene 1, EDG 1, EDG-1, Endothelial Differentiation Sphingolipid G Protein Coupled Receptor 1, CHEDG1, D1S3362, G Protein Coupled Sphingolipid Receptor, Sphingosine 1-phosphate Receptor Edg-1, Sphingosine 1-phosphate R

Anti -EDG1 (Endothelial Cell Differentiation Gene 1, EDG 1, EDG-1, Endothelial
Differentiation Sphingolipid G Protein Coupled Receptor 1, CHEDG1, D1S3362, G Protein
Coupled Sphingolipid Receptor, Sphingosine 1-phosphate Receptor Edg-1, Sphingosine
1-phosphate R

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
Movement of lymphocytes through lymphoid organs is required for generating immunity. Their migration into lymph nodes follows a series of events including integrin activation through chemokine signaling, adhesion and diapedis. The release of lymphocytes from lymph nodes is regulated by the phospholipid sphingosine-1-phosphate (S1P). One of its receptors S1P1 binds S1P with high specificity and affinity; agonism of this receptor by the immunosuppressive agent FTY720 inhibits the entry of tissue T cells into afferent lymphatics in homeostatic and inflammatory conditions. Recent experiments have indicated that CCR7-deficient T cells left lymph nodes more rapidly than wild-type cells did and these cells where also less effectively retained after treatment with FTY720, and that egress competence could be restored by inactivating G alpha i-protein-coupled receptor signaling. These results suggest that S1P1 acts in the lymphocyte to promote lymph node egress by overcoming retention signals mediated by CCR7 and G alpha i-protein-coupled receptor signaling. At least two isoforms of S1P1 are known to exist; this S1P1 antibody will only recognize the shorter isoform.
Catalog #E2208-01G
ApplicationsSuitable for use in Elisa and Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1-2ug/ml
Optimal dilutions to be determined by the researcher.
Positive ControlMouse Thymus Tissue Lysate
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostRabbit
SourceHuman
ConcentrationAs reported
FormSupplied as a liquid in PBS, 0.02% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to 14aa from near the carboxy terminus of the human S1P1.
SpecificityRecognizes human S1P1. Species Crossreactivity: Mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links