EOMES is a 72kD member of the TBR1 subfamily, T-box family of transcription factors. It is expressed in NK and CD8+ T cells, where CTLA4 activation suppresses EOMES activation of IFN-g and granzyme B genes. It is also found in the embryo, where it occurs in forebrain floorplate and migrating neuroblasts at 12.5 weeks gestation. Notably, it is reported to undergo intercellular transfer in fetal Xenopus tissue destined to become mesoderm. Here, it synchronizes a multicellular commitment to a cell lineage.
Applications
Suitable for use in ELISA, Western Blot, Flow Cytometry and Immunocytochemistry. Other applications not tested.
Recommended Dilutions
Western Blot: 1ug/ml Immunocytochemistry: 8-25ug/ml Flow Cytometry: 2.5ug/10e6 cells Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant protein corresponding to Gly471-Pro686 from human EOMES expressed in E. coli.
Form
Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
Purity
Purified by Protein A affinity chromatography from hybridoma culture supernatant.
Specificity
Recognizes human EOMES. No cross-reactivity with recombinant human Brachyury, rhEOMES (aa1-115), recombinant mouse EOMES (aa1-126), rhTBX2, 3, 5, 6, 18, or 20.
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