Applications
Suitable for use in Flow Cytometry, ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended Dilutions
Flow Cytometry: 0.25ug/ml labels 10e6 of COLO 205 human colorectal adenocarcinoma cell line and D3 mouse embryonic stem cell line Western Blot: 0.1ug/ml Immunohistochemistry: 1-15ug/ml using immersion fixed frozen sections of embryonic mouse brain and mouse embryo and paraffin sections of human esophageal squamous cell Immunocytochemistry: 5-15ug/ml of immersion fixed MDA-MB-468 human breast cancer cel line Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant protein corresponding to Val27-Lys548 of mouse EphB2 expressed in NS0 cells.
Form
Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes mouse EphB2. Species Crossreactivity: human. In Western Blots, this antibody shows ~5% crossreactivity with rrEphB1, rmEphA8, rmEphA6, rmEphB6, rmEphA3, rmEphA4, rmEphA7, rrEphA5, rhEphA1, rmEphA2 and rmEphB3.