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E3414-61A Rabbit Anti-Epithelial Sodium Channel, alpha (ENAC Alpha)

Specifications
References
Clone Type
Polyclonal
Host
Rabbit
Source
Rat
Isotype
IgG
Grade
Affinity Purified
Applications
E
Crossreactivity
Rt
Shipping Temp
Blue Ice
Storage Temp
4°C (-20°C Glycerol)

Tissue acidosis (decrease in pH below the physiological level) that occurs in ischemia, tissue damage or inflammation is accompanied by pain. At the molecular level, H+-gated cation channels are activated by low pH in nociceptive neurons. H+-gated cation channels, members of the NaC/DEG superfamily of Na channels that include amiloride-sensitive epithelial Na+ channel proteins (alpha, beta, gamma and delta-ENaC subunits), are expressed in epithelia of the vertebrate kidney, colon, lung, tongue and brain. The ENaC subunits may form heterotrimeric active sodium channel. ENaCs are involved in sodium and water readsorption, and salty taste transduction. The superfamily of DEG/NaC proteins are characterized by intracellular N and C-termini, two TM domains, and a large extracellular loop.

ENaC alpha subunits have been cloned from various tissues and from several species (human 669aa, rat 698aa, mouse 699aa; ~80% inter-species homology). It controls the reabsorption of sodium in the kidney, colon, lung and sweat glands. ENaC alpha has also been implicated in taste perception. Defects in ENaC alpha are one of the cause of pseudohypoaldosteronism type I, a rare salt wasting disease characterized by dehydration, hyponatacemia, hyperkalemia and acidosis. Inactivation of ENaC alpha in transgenic mice causes an early death due to defective lung liquid clearance.
Applications
Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Note: Because of its low MW (< 3kD). The control peptide is not suitable for Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 1-10ug/ml using ECL.
ELISA: 0.5-1ug/ml. Control peptide can be used to coat ELISA plates at 1ug/ml. See E3414-61F.
Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
A 20aa peptide sequence near the N-terminus of rat ENAC alpha
Form
Supplied as liquid in Tris, pH 7.5, 0.2% BSA, 0.05% sodium azide, 40% glycerol.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes rat Epithelial Sodium Channel, alpha (ENAC alpha). The 20aa rat control peptide sequence homology is: 90% in mouse, 75% in human, 70% in rabbit, 65% in bovine. No significant homology is detected with ENAC beta, gamma or delta subunits.
References
1. Linguela, E., et al., (1993) FEBS Lett. 318:95-99. 2. Canessa, C.M., et al., (1993) Nature 361:467-470. 3. Snyder, P.M., et al., (1994) J. Biol. Chem. 269:24,379-24,383. 4. Waldmann, R., et al., (1995) J. Biol. Chem. 270:11,735-11,737. 5. Kreutz, R., et al., (1997) Hypetension 29:131-136. 6. Voilley, N., et al., (1994) Proc. Natl. Acad. Sci. 91:247-251. 7. McDonald, F.J., et al., (1994) Am. J. Physiol. 266:L728-L734. 8. Ludwig, M., et al., (1998) Human Genet. 102:576-581. 9. Garty, H. and Palmer, L.G., (1997) Physiol. Reviews 77:359-396 (review). 10. Willis, (2003) Am. J. Physiol. Renal Physiol. 285:1,192-1,200. 11. Schmitt, R., (2003) Am. J. Physiol. Renal Physiol. 284:1,097-1,104
USBio References
No references available
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