Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications Monoclonal Mouse Supernatant B IP IH

Catalog #	E3564-82
Applications	Suitable for use in Immunoprecipitation, Immunohistochemistry (paraffin sections), and Western Blot. Other applications have not been tested.
Recommended Dilution	Immunohistochemistry (formalin/paraffin): 1:40-1:80 for 30 mins. at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min., followed by cooling at RT for 20 min.
	Immunoprecipitation (denatured verified): 10ul/mg protein lysate. Use Protein G.
	Western Blot: 1:50-1:100 for 2 hrs. at RT
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone Type	Monoclonal
Isotype	IgG1
Clone No	0.N.289
Host	Mouse
Source	Human
Concentration	Not determined
Form	Supplied as concentrated tissue culture supernatant in 15mM sodium azide.
Purity	Tissue culture supernatant
Immunogen	Recombinant full length human estrogen receptor protein.
Specificity	Recognizes human Estrogen Receptor at 67kDa. Species Reactivity: Rat.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.