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You are here:Home » Antibodies » Antibodies-Receptors, Estrogen » Anti -Estrogen Receptor (ER)

Anti -Estrogen Receptor (ER)


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Clone Host Grade Applications
Monoclonal Mouse Supernatant B IP IH
Catalog #E3564-82
ApplicationsSuitable for use in Immunoprecipitation, Immunohistochemistry (paraffin sections), and Western Blot. Other applications have not been tested.
Recommended DilutionImmunohistochemistry (formalin/paraffin): 1:40-1:80 for 30 mins. at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min., followed by cooling at RT for 20 min.
Immunoprecipitation (denatured verified): 10ul/mg protein lysate. Use Protein G.
Western Blot: 1:50-1:100 for 2 hrs. at RT
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
Clone No0.N.289
ConcentrationNot determined
FormSupplied as concentrated tissue culture supernatant in 15mM sodium azide.
PurityTissue culture supernatant
ImmunogenRecombinant full length human estrogen receptor protein.
SpecificityRecognizes human Estrogen Receptor at 67kDa. Species Reactivity: Rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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