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You are here:Home » Antibodies » Abs to Translation Initiation Factors » Anti -Eukaryotic Translation Initiation Factor 4B (eIF4B)

Anti -Eukaryotic Translation Initiation Factor 4B (eIF4B)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified B
eIF-4B (eukaryotic translation initiation factor 4B),an RNA binding protein, is essential for the binding of mRNA to the 43S pre-initiation complex, which consists of the 40S ribosomal subunit bound to a ternary complex of eIF-2, GTP, and Met-tRNA stabilized by eIF-3. An arginine rich motif (ARM) in the carboxy-terminus of eIF-4B binds RNA non-specifically, while a canonical RNA motif (RMM) near the amino terminus binds specifically to 18S rRNA. The simultaneous binding of specific and nonspecific RNA may serve to facilitate the binding of the 40S subunit to the mRNA by serving as a bridge between the 18S rRNA and the mRNA. Besides its binding activity, eIF-4B in conjunction with eIF-4F, a heterotrimeric protein, stimulates the ATPase and RNA helicase activity of eIF-4E.
Catalog #E8949-38T
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 0.5ug/ml
Optimal dilution determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute by adding sterile 40-50% glycerol, PBS, 0.02% sodium azide. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
Concentration~1mg/ml
FormSupplied as a lyophilized powder in PBS, 0.02% sodium azide. Reconstitute with 100ul sterile 40-50% glycero, PBS, 0.02% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenE. coli-derived recombinant human eIF-4B (rheIF-4B, aa1-200)
SpecificityRecognizes human eIF4B. Species Crossreactivity: mouse
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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